ANGPT2单克隆抗体对AML小鼠肿瘤组织血管增生和下游信号蛋白表达的影响

Effects of monoclonal antibody ANGPT2 on vascular hyperplasia and downstream signaling protein expression in tumor tissues of AML mice

目的 探讨ANGPT2单克隆抗体对急性髓系白血病(AML)小鼠肿瘤组织血管增生、下游信号蛋白表达的影响。方法 建立AML小鼠模型20只,对照组与实验组各10只,采用免疫组织化学方法检测微血管密度及VEGF表达情况,采用Western blot检测下游信号通路蛋白TIE2、SHP2和PI3K的表达水平。结果 ANGPT2单克隆抗体治疗后,AML小鼠肿瘤组织微血管密度及VEGF表达水平显著下降79.29%(P<0.01),下游信号通路蛋白SHP2蛋白表达水平上升59.43%,PI3K蛋白表达水平下降20.37%(P<0.01)。结论 ANGPT2单克隆抗体显著抑制AML小鼠肿瘤组织血管增生。

Objective To investigate the effects of monoclonal antibody ANGPT2 on vascular proliferation and downstream signaling protein expression in tumor tissues of AML mice. Methods After establishing the AML mouse model,immunohistochemistry was used to detect the microvascular density and VEGF expression,and Western blot was used to detect the expression levels of downstream signaling pathway proteins TIE2,SHP2 and PI3K.Results After treatment with ANGPT2 monoclonal antibody,the microvascular density and VEGF expression level in tumor tissues of AML mice were significantly decreased,and the expression level of SHP2 protein in downstream signaling pathway was increased,while PI3K protein expression level was decreased. Conclusion ANGPT2 monoclonal antibody can significantly inhibit angiogenesis in tumor tissues of AML mice,suggesting its potential application in the clinical treatment of AML in the future,and providing a theoretical basis for targeted therapy of AML.