响应面优化超声波辅助提取杏鲍菇多糖的工艺及其抗疲劳活性测定研究

Optimization of ultrasonic assisted extraction of pleurotus eryngii polysaccharide by response surface methodology and its antifatigue activity

本试验以杏鲍菇为原料,采用超声波辅助提取杏鲍菇多糖,并对其抗疲劳作用进行研究。以杏鲍菇多糖提取率为指标,在单因素基础上进行响应面优化提取工艺,得到最佳工艺条件为:超声功率420 W、超声温度50℃、超声时间50min,在此条件下,杏鲍菇多糖提取率为14.71%。观察小鼠的爬杆和负重游泳时间、血清生化指标、肝糖原、肌糖原等指标考察杏鲍菇多糖的抗疲劳作用。结果表明,杏鲍菇多糖能明显延长爬杆和游泳时间,提高SOD活性、降低LA含量,提高肝糖原和肌糖原含量,表明杏鲍菇多糖具有抗疲劳作用。

Pleurotus eryngii was extracted by ultrasonic-assisted extraction and its anti-fatigue effect was studied.The extraction rate of pleurotus eryngii polysaccharide was used as the index,extraction process was optimized by response surface method on the basis of single factor experiment. The optimum conditions were as follows:ultrasonic power 420 W,ultrasonic temperature 50 ℃ and ultrasonic time 50 min. Under the above conditions,the extraction rate of pleurotus eryngii polysaccharide was 14.71%. The anti-fatigue effect of pleurotus eryngii polysaccharide was measured by observing the time of mice climbing and weight-bearing swimming,serum biochemical indexes,and liver glycogen and myoglycogen. The results showed that pleurotus eryngii polysaccharide could significantly increase the climbing and swimming time of mice,SOD activity,reduce La content,and increase the content of liver glycogen and muscle glycogen,which indicating that pleurotus eryngii polysaccharide has anti-fatigue effect.