SMARCA5、TGFBRI在结直肠癌转移中的作用及临床意义

The role and clinical significance of SMARCA5 and TGFBRI in colorectal cancer metastasis

目的探讨SMARCA5、转化生长因子-β1受体(TGFBRI)在结直肠癌转移患者中的作用及临床意义。方法回顾性选取2019年1月到2022年5月在潜江市中心医院及华中科技大学同济医学院附属协和医院进行手术治疗的190例结直肠癌患者为研究对象,并在术中收集患者的癌组织标本及癌旁组织标本,采用免疫组织化学染色法观察结直肠癌转移组织中SMARCA5、TGFBRI表达情况;采用多因素Logistic回归风险模型分析结直肠癌转移的影响因素;利用Kaplan-Meier法绘制生存曲线。结果结直肠癌转移组织中的SMARCA5阳性表达率为22.47%,低于未转移组织(36.63%)和正常上皮组织(82.18%),TGFBRI在结直肠癌转移组织中的阳性表达率为78.65%,高于未转移组织(65.35%)和正常上皮组织(14.85%),差异均有统计学意义(P<0.05)。单因素分析结果显示,分化程度、神经侵犯、肿瘤浸润、肿瘤直径、AJCC分期、SMARCA5与TGFBRI表达均与结直肠癌转移的发生相关(P<0.05);多因素Logistic回归分析结果显示分化程度、神经侵犯、肿瘤浸润、AJCC分期、SMARCA5阴性表达、TGFBRI阳性表达均是影响结直肠癌转移的危险因素(P<0.05)。Kaplan-Meier分析结果显示,SMARCA5阳性表达患者3年累积生存率为78.95%,显著高于阴性表达患者(48.12%),差异有统计学意义(P<0.05);TGFBRI阳性表达患者3年累积生存率为48.53%,显著低于阴性表达患者(79.63%),差异有统计学意义(P<0.05)。结论SMARCA5在结直肠癌转移患者癌组织中表达降低,TGFBRI表达升高,二者表达与结直肠癌转移的发生及预后关系密切。

Objective To investigate the role and clinical significance of SMARCA5 and transforming growth factor-β1 receptor-1(TGFBRI)in patients with colorectal cancer metastasis.Methods A total of 190 patients with colorectal cancer who underwent surgical treatment in Qianjiang Central Hospital and Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology from January 2019 to May 2022 were regarded as the research objects.The patient’s cancer tissue specimens and paracancerous tissue specimens were collected during the operation.Immunohistochemical staining was used to observe the expression of SMARCA5 and TGFBRI in colorectal cancer metastatic tissue;multivariate logistic regression risk model was applied to analyze the influencing factors of colorectal cancer metastasis;survival curves were drawn using the Kaplan-Meier method.Results The positive expression rate of SMARCA5 in colorectal cancer metastatic tissue was 22.47%,which was lower than those in non-metastatic tissue(36.63%)and normal epithelial tissue(82.18%),the positive expression rate of TGFBRI in colorectal cancer metastatic tissues was 78.65%,which was higher than those in non-metastatic tissues(65.35%)and normal epithelial tissues(14.85%),the differences were statistically significant(P<0.05).Univariate analysis showed that the degree of differentiation,nerve invasion,tumor infiltration,tumor diameter,AJCC stage,SMARCA5 and TGFBRI expressions were all associated with the occurrence of colorectal cancer metastasis(P<0.05).Multivariate Logistic regression analysis showed that the degree of differentiation,nerve invasion,tumor infiltration,AJCC stage,negative expression of SMARCA5 and positive expression of TGFBRI were all risk factors for colorectal cancer metastasis(P<0.05).Kaplan-Meier analysis showed that the 3-year cumulative survival rate of patients with positive SMARCA5 expression was 78.95%,which was obviously higher than that of patients with negative expression(48.12%),the difference was statistically significant(P<0.05);the 3-year cumulative survival rate of patients with positive TGFBRI expression was 48.53%,which was obviously lower than that of patients with negative expression(79.63%),the difference was statistically significant(P<0.05).Conclusion The expression of SMARCA5 is decreased in the cancer tissues of patients with colorectal cancer metastasis,while the expression of TGFBRI is increased,and the expressions of the two are closely related to the occurrence and prognosis of colorectal cancer metastasis.