慢性乙肝患者肝纤维化严重程度与血清TGF-β的相关性分析

Correlation Analysis of Severity of Liver Fibrosis and Serum TGFβin Patients with Hepatitis B Virus

目的分析慢性乙肝患者血清转化生长因子-β(TGF-β)水平与肝纤维化参数指标的关系。方法将中国科学院大学深圳医院(光明)感染性疾病科2019年5月—2022年1月收治的296例慢性乙肝患者作为观察组,以及中国科学院大学深圳医院(光明)感染性疾病科2019年5月—2022年1月门诊体检的296名健康人员作为对比组,均采集空腹静脉血,检测TGF-β、Ⅳ型胶原(C-Ⅳ)、层黏蛋白(LN)、Ⅲ型前胶原(PC-Ⅲ)、透明质酸(HA)。对比两组肝纤维化实验室参数、血清TGF-β,对比观察组患者中轻度组(30例)、中度组(157例)、重度组(109例)血清TGF-β、肝纤维化指标,对比观察组患者中S0期组(21例)、S1-2期组(131例)、S3-4期组(144例)血清TGF-β、肝纤维化指标,Spearman相关性分析C-Ⅳ、LN、PC-Ⅲ、HA与TGF-β的相关性。结果观察组血清TGF-β、PC-Ⅲ、C-Ⅳ、LN、HA均高于对比组,差异有统计学意义(P<0.05)。不同严重程度组血清TGF-β、LN、HA、PC-Ⅲ、C-Ⅳ比较,重度组高于中度组,中度组高于轻度组,差异有统计学意义(P<0.05)。S3-4期组血清TGF-β、LN、HA、PC-Ⅲ、C-Ⅳ均高于S1-2期,S1-2期血清TGF-β、LN、HA、PC-Ⅲ、C-Ⅳ均高于S0期组,差异有统计学意义(P<0.05)。LN、HA、PC-Ⅲ、C-Ⅳ与TGF-β均呈正相关(r=4.937、4.968、4.829、5.024,P<0.05)。结论肝纤维化程度越重的慢性乙肝患者,血清TGF-β浓度越高,两者之间呈正相关性,临床可通过检测以上指标,评估病情严重程度及预后。

Objective To analyze the relationship between serum transforming growth factor-β(TGF-β)levels and liver fibrosis parameters in hepatitis B virus patients.Methods Two hundred and ninety-six chronic hepatitis B pa⁃tients admitted to the Department of Infectious Diseases,Shenzhen Hospital of the Chinese Academy of Sciences(Guangming)from May 2019 to January 2022 were used as the observation group,and 296 healthy individuals from the Department of Infectious Diseases,Shenzhen Hospital of the Chinese Academy of Sciences(Guangming)from May 2019 to January 2022 were used as the comparison group.Fasting venous blood was collected in all cases,and TGF-β,collagen typeⅣ(C-Ⅳ),laminin(LN),precollagen typeⅢ(PC-Ⅲ),and hyaluronic acid(HA)were detected.The laboratory parameters of liver fibrosis and serum TGF-βwere compared between the two groups.Compare serum TGF-β,liver fibrosis indexes in the mild group(30 cases),moderate group(157 cases)and severe group(109 cases)in the observation group.Serum TGF-βand liver fibrosis indexes in the S0 group(21 cases),S1-2 group(131 cases)and S3-4 group(144 cases)were compared among the patients in the observation group.Spearman correlation analy⁃sis of C-Ⅳ,LN,PC-Ⅲ,HA and TGF-βwas performed.Results Serum TGF-β,PC-Ⅲ,C-Ⅳ,LN and HA in the ob⁃servation group were higher than the in the comparison group,the difference was statistically significant(P<0.05).When comparing serum TGF-β,LN,HA,PC-Ⅲ,and C-Ⅳin different severity groups,the severe group was higher than the moderate group,and the moderate group was higher than the mild group,the difference was statistically sig⁃nificant(P<0.05).Serum TGF-β,LN,HA,PC-Ⅲ,and C-Ⅳin the S3-4 stage group were higher than those in the S1-2 stage.Serum TGF-β,LN,HA,PC-Ⅲ,and C-Ⅳin the S1-2 stage were all higher than those in the S0 stage group,the difference was statistically significant(P<0.05).LN,HA,PC-Ⅲ,C-Ⅳand TGF-βwere positively correlated(r=4.937,4.968,4.829,5.024,P<0.05).Conclusion The more severe the degree of liver fibrosis in hepatitis B virus pa⁃tients,the higher the serum TGF-βconcentration,and there was a positive correlation between the two,and the clini⁃cal can assess the severity and prognosis of the disease by testing the above indexes.