脊髓脱细胞基质材料搭载hMSCs及bFGF治疗大鼠脊髓损伤效果

EFFECT OF SPINAL CORD DECELLULARIZED MATRIX CARRYING HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS AND BASIC FIBROBLAST GROWTH FACTOR IN TREATMENT OF RATS WITH SPINAL CORD INJURY

目的观察脊髓脱细胞基质(SCDM)材料搭载人脐带间充质干细胞(hMSCs)及碱性成纤维生长因子(bFGF)对大鼠脊髓损伤(SCI)的修复效果。方法制备SCDM并检测其特性及生物相容性;对hMSCs及bFGF进行体外细胞活性检测。将实验大鼠分为对照组、SCDM组、SCDM+hMSCs组和SCDM+hMSCs+bFGF组,4组大鼠均行脊髓完全横断损伤,后3组SCI后给予相应治疗。术后评价大鼠运动功能,4周后取出脊髓进行苏木精-伊红(HE)染色及相关免疫荧光染色。结果SCDM+hMSCs+bFGF组大鼠术后运动功能评分显著高于对照组及SCDM组。HE染色结果显示,4组大鼠脊髓组织均有炎症细胞浸润,其中对照组大鼠炎症细胞浸润最明显。SCDM+hMSCs+bFGF组损伤中心Tuj1、Nestin免疫荧光染色强度高于其余各组,而GFAP免疫荧光强度低于对照组及SCDM组。结论该复合生物支架材料具有促进大鼠SCI修复的作用。

Objective To investigate the effect of spinal cord decellularized matrix(SCDM)carrying human mesenchymal stem cells(hMSCs)and basic fibroblast growth factor(bFGF)on the repair of spinal cord injury(SCI)in rats.Methods SCDM was prepared and then tested in terms of characteristics and biocompatibility,and in vitro cell survival assay was performed for hMSCs and bFGF.Experimental rats were divided into control group,SCDM group,SCDM+hMSCs group,and SCDM+hMSCs+bFGF group;the rats in all four groups had complete spinal cord transection injury,and those in the latter three groups were given corresponding treatment after SCI.Motor function was evaluated after surgery,and the spinal cord was collected after four weeks for HE staining and immunofluorescent staining.Results The SCDM+hMSCs+bFGF group had a significantly higher motor function score than the control group and the SCDM group.HE staining showed inflammatory cell infiltration in all four groups,with the most significant inflammatory cell infiltration in the control group.The SCDM+hMSCs+bFGF group had a significantly higher immunofluorescence intensity of Tuj1 and Nestin in the center of injury than the other three groups,as well as a significantly lower immunofluorescence intensity of GFAP than the control group and the SCDM group.Conclusion This composite biological scaffold material can promote the repair of SCI in rats.