33份旱黄瓜种子萌发期抗旱性鉴定

Analysis on drought resistance of 33 cucumber seeds at germination stage

为研究旱黄瓜种子萌发期的抗旱性,以33份旱黄瓜品种资源为试材,利用15%PEG-6000模拟干旱环境,统计种子相对发芽率(RGR)、相对发芽势(RGE)、萌发抗旱指数(GDRI)、萌发胁迫指数(GSI)、活力抗旱指数(VDRI)等指标,并采用模糊隶属函数法进行抗旱性综合评价。结果表明,供试黄瓜种子相对发芽势、相对发芽率、萌发抗旱指数、胁迫指数及活力抗旱指数与隶属排名较一致,均可作为评价黄瓜种子萌发期抗旱性指标;将隶属值≥0.6判定为高抗材料,0.6>隶属值≥0.2判定为中抗材料,隶属值<0.2判定为弱抗材料,可作为评价黄瓜抗旱性的重要参数;筛选出高抗旱品种4个、弱抗品种6个和中抗品种23个。该研究为黄瓜抗旱性鉴定和评价提供可靠的指标和方法,为黄瓜抗旱育种提供种质资源和理论依据。

To study the drought resistance of the soil cucumber seed germination,a total of 33 cucumber variety were as materials,using 15%PEG-6000 simulated drought environment,to statistical relative seed germination rate(RGR),relative germination energy(RGE),germination index of drought resistance(GDRI),stress of germination index(GSI)and vigor index of drought index(VDRI),and drought resistance comprehensive evaluation by fuzzy membership function method.The results showed that the value of RGR,RGE,GDRI,GSI and VDRI were consistent with the ranking of subordinate function value,which could be used to evaluate the drought resistance of cucumber seeds at germination stage.The membership function value≥0.6 was judged as high resistance material,0.6>The membership function value≥0.2 was judged as medium resistance material,and the membership function value<0.2 was judged as weak resistance material,which could be used as an important parameter to evaluate drought resistance of cucumber.Four high resistance cultivars,six weak resistance cultivars and 23 medium resistance cultivars were identified.These results provided reliable indexes and methods for the identification and evaluation of drought resistance of cucumber and germplasm resources and theoretical basis for drought resistance breeding of cucumber.