富氢水对草莓生长发育及光合作用的影响

Effects of hydrogen-rich water on growth and photosynthesis of strawberry

[目的]本文旨在探究富氢水对草莓(Fragaria×ananassa Duch.)生长和光合作用的调控及其作用机制,为富氢水在农业生产中的应用提供依据。[方法]用富氢水(hydrogen-rich water,HRW)灌溉草莓,测定草莓生长、光合气体交换和光合效率等参数。以地表水灌溉为对照。[结果]HRW处理后草莓叶面积增加,叶片干重、鲜重分别增加57.7%和60.4%,相对生长率和净同化率分别增加50.0%和59.9%,表明HRW对草莓生长有明显促进作用。HRW处理后叶绿素a含量增加14.4%、叶绿素b含量无明显变化,叶片净光合速率提高22.3%,叶片可溶性糖、葡萄糖和蔗糖含量显著增加。HRW处理后,经过PSⅡ的电子传递速率增加但不显著,而光合系统Ⅱ非调节性能量耗散的量子产量Y(NO)的显著降低表明草莓叶片光合机构保护调节能力提高;经过PSⅠ的电子传递速率及有效光化学量子产量Y(Ⅰ)显著增加,暗示PSⅠ环式电子流的提高。进一步分析发现跨膜质子梯度(ΔpH)显著高于对照,占跨膜质子动力势的主要部分,ATP含量较对照增加43.9%,验证环式电子流增加的结果。[结论]富氢水通过增强环式光合电子流促进草莓叶片的光合作用和生长。

[Objectives]The purpose of this study was to investigate the regulation and mechanism of hydrogen-rich water(HRW)on the growth and photosynthesis of strawberry(Fragaria×ananassa Duch.).This would benefit agriculture by the application of HRW.[Methods]HRW was used to irrigate strawberries,and parameters such as leaf growth,photosynthetic gas exchange and photosynthetic efficiency were determined.Surface water irrigation was as the control.[Results]After the treatment,the leaf area of strawberries increased,the dry weight and fresh weight of leaves increased by 57.7%and 60.4%,respectively,and the relative growth rate and net assimilation rate increased by 50.0%and 59.9%,respectively,indicating that HRW had a significant effect in promoting strawberry growth.After HRW treatment,the chlorophyll a content increased by 14.4%,the chlorophyll b content did not change significantly,and the net photosynthetic rate of leaves increased by 22.3%,the contents of soluble sugar,glucose and sucrose in leaves significantly increased.After HRW treatment,the electron transport rate of PSⅡincreased but was not significant,while the significant decrease of quantum yield of photosystemsⅡnonregulatory energy dissipation Y(NO)indicated that the protection and regulation ability of the PSⅡof strawberry leaves was improved.While the electron transport rate of PSⅠand effective photochemical quantum yield Y(I)significantly increased,suggesting that the cyclic electron flow of PSⅠwas improved.Further analysis showed that the transmembrane proton gradient(ΔpH)was significantly higher than that of the control,accounting for the main part of proton motive force,and the ATP content increased by 43.9%compared with the control,confirming the cyclic electron flow results.[Conclusions]Hydrogen-rich water can promote growth and photosynthesis of strawberry leaf by enhancing cyclic photosynthesis electron flow.