东北三省和浙江木耳稳定同位素特征与产地溯源

Stable Isotope Characteristics and Origin Traceability of Auricularia auricula in Three Northeast Provinces and Zhejiang Province

为探究稳定同位素在黑木耳产地溯源中的可行性,本研究从东北(黑龙江、吉林、辽宁)、浙江产地采集74份代表性黑木耳样品,从新疆采集11份代表性黑木耳样品作为外部验证,采用元素分析-稳定同位素比率质谱仪测定δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值,结合化学计量学方法进行产地溯源判定。结果表明,东北黑木耳δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值分别为-24.5‰~-22.7‰、-0.9‰~3.1‰、-62.2‰~-34.6‰、15.9‰~19.4‰;浙江黑木耳δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值分别为-26.2‰~-24.5‰、-0.9‰~1.2‰、-24.9‰~-9.0‰、19.9‰~22.2‰,两产地黑木耳δ^(15)N值差异不显著(P>0.05),δ^(13)C、δ^(2)H和δ^(18)O值均呈现显著差异(P<0.05)。偏最小二乘法判别分析结果表明,东北(黑龙江、吉林、辽宁)黑木耳判别模型训练集总体判别率为98.4%,验证集总体判别率为95.2%;浙江黑木耳判别模型训练集总体判别率为100%,验证集总体判别率为100%,具有较好的判别准确性。综上所述,稳定同位素分析结合化学计量学模型能够较好区分东北(黑龙江、吉林、辽宁)和浙江黑木耳,对不同黑木耳进行产地溯源和判别,具有一定的应用价值。

In order to explore the feasibility of stable isotope in the traceability of Auricularia auricula,74representative Auricularia auricula samples were collected from Northeast China(Heilongjiang,Jilin,Liaoning)and Zhejiang,and 11 representative Auricularia auricula samples were collected from Xingjiang as external verification.δ^(13)C value,δ^(15)N value,δ^(2)H value and δ^(18)O value were analyzed by elemental analysis-stable isotope ratio mass spectrometer,and the traceability of Auricularia auricula was determined by chemometrics.The results showed that the range ofδ^(13)C was between-24.5‰and-22.7‰,δ^(15)N was between -0.9‰ and 3.1‰,δ^(2)H was between -62.2‰ and -34.6‰,and δ^(18)O was between 15.9‰ and 19.4‰ for samples from Northeast China(Heilongjiang,Jilin,Liaoning).And the range of δ^(13)C was from -26.2‰ to -24.5‰,δ^(15)N was from -0.9‰ to 1.2‰,δ^(2)H was from -24.9‰ to -9.0‰,and δ^(18)O was from 19.9‰ to 22.2‰ for samples from Zhejiang.There was no significant difference in δ^(15)N between the two habitats(P>0.05),while δ^(13)C,δ^(2)H and δ^(18)O showed significant differences(P<0.05).Partial least square discriminant analysis showed that the overall discriminant rate of the Northeast China(Heilongjiang,Jilin,Liaoning)Auricularia auricula was 98.4%for the training set of discriminant model,and the overall rate was 95.2% for the verification set.The overall discriminant rate of Zhejiang Auricularia auricula was 100%for the discriminant model training set,and the overall discriminant rate of verification set was also 100%,which had good discriminant accuracy.To sum up,stable isotope analysis combined with chemometrics model could well distinguish Auricularia auricula from Northeast China(Heilongjiang,Jilin,Liaoning)and Zhejiang Province,and can trace the origin of different Auricularia auricula,which has certain application value.