槲皮素对α-ZOL诱导Caco-2细胞损伤的保护作用研究

Protective Effect of Quercetin on α-ZOL-induced Injury of Caco-2 Cells

为研究槲皮素对α-玉米赤霉烯醇(α-ZOL)诱导细胞损伤的保护作用,通过研究活性氧水平、线粒体膜电位、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力、三磷酸腺苷(ATP)含量等变化情况,分析槲皮素对α-ZOL诱导的细胞氧化损伤和线粒体损伤的影响;通过荧光染色和流式细胞术检测研究细胞凋亡情况,并利用蛋白免疫印迹法检测目的蛋白核转录因子E2相关因子2(Nrf2)、B淋巴细胞瘤-2(Bcl-2)、半胱天冬酶-3(Caspase-3)的表达。结果表明,模型组人结肠腺癌细胞Caco-2细胞氧化损伤程度随α-ZOL浓度的递增而加剧;与模型组相比,槲皮素可通过降低细胞内活性氧、MDA含量和提高抗氧化酶活力来减少α-ZOL对Caco-2细胞的氧化损伤,并通过提高ATP含量和线粒体膜电位来改善线粒体功能。此外,槲皮素还会上调Nrf2和Bcl-2的表达、下调Caspase-3的表达,从而减少由α-ZOL引起的细胞凋亡。本研究结果对预防和控制α-ZOL的毒性具有积极意义。

To understand the protective effect of quercetin on α-ZOL-induced damage to Caco-2 cells,the effects of quercetin on the oxidative damage and mitochondrial damage were analyzed using reactive oxygen species,mitochondrial membrane potential,malondialdehyde,superoxide dismutase activity and ATP content.AO fluorescence staining and flow cytometry were used to detect apoptosis.The relative expression levels of Nrf2,Bcl-2,Caspase-3 protein were determined by western blot.Results indicated that the degree of oxidative damage of Caco-2 cells increased with the α-ZOL concentration.Compared with model group,quercetin reduced the oxidative stress of α-ZOL on Caco-2 cells by decreasing the level of reactive oxygen species and malondialdehyde,as well as increasing the activity of antioxidant enzymes.The mitochondrial function can be improved by increasing ATP levels and mitochondrial membrane potential.Additionally,by up-regulating the expression of Nrf2 and Bcl-2,as well as down-regulating the expression of Caspase-3,quercetin can reduce apoptosis induced byα-ZOL(P<0.05).The results could be used to prevent and control the toxicity of α-ZOL.