表面活性剂协同超声提取香菇多糖

The Extraction of Lentinan by Surfactant Synergistic Ultrasound

以香菇为原料,探究表面活性剂协同超声优化提取香菇多糖,以香菇多糖含量为指标,通过单因素试验,研究表面活性剂添加量、料液比和超声处理时间三个因素对香菇中多糖含量的影响,在单因素试验基础上,利用三因素三水平的Box-Behnken试验设计及响应面分析方法对香菇多糖提取工艺进行优化。结果表明:最佳提取工艺条件为聚乙二醇-400添加量2%、超声处理时间40 min、料液比1∶20(g/mL);此工艺条件下,香菇多糖含量达到7.05%,该结果与建立模型预测值7.247%基本相符。

To optimize the extraction of lentinan by surfactant-assisted ultrasonication extration by using lentinan edodes as raw material,with the content of lentinan as the index,the extraction process of Lentinan was optimized by single factor experiment,a three-factor and three-level Box-Benhnken experiment design and response surface analysis.The results showed that the optimun extraction conditions were 2%addition of polyethylene glycol-400,40 min of extractive time and 1∶20(g/m L)of material-liquid ratio.Under these conditions,the content of Lentinan reached 7.05%,which was basically consistent with the predicted value of 7.24%.