高效液相色谱法快速测定白酒中纽甜的含量

Rapid Determination of Neotame in Liquor by High Performance Liquid Chromatography

研究建立了高效液相色谱法快速测定白酒中纽甜的含量,简化了国标中对样品的前处理过程,优化了色谱条件。白酒除乙醇后用水定容上机,采用Thermo C_(18)色谱柱,以乙腈-辛烷磺酸钠溶液=40∶60(V/V)为流动相,采用二极管阵列检测器,检测波长210 nm,柱温30℃,流速1 mL/min。结果表明,在该色谱条件下,纽甜在0.2~10 mg/L的质量浓度范围内与峰面积具有良好的线性关系,相关系数R值为0.999978,方法检出限为0.06 mg/kg,定量限为0.2 mg/kg,3个不同添加量0.2,0.5和1.0 mg/kg水平下纽甜加标平均回收率为97.1%~99.0%,相对标准偏差(n=6)为0.39%~1.3%。该方法前处理简单、快速,在该色谱条件下分离效果好,整个检验过程分析成本低,为检测白酒中的纽甜含量提供了更快捷的方法。

A high performance liquid chromatography method was established for the rapid determination of neotame in liquor,which simplified the pretreatment process of the samples in the national standard and optimized the chromatographic conditions.After removing the ethanol,the liquor was directly connected to the machine,neotame was qualitatively analyzed on a Thermo C_(18)column with a diode array detector at 210 nm using acetonitrile-sodium octane sulfonate solution of 40∶60(V/V)as the mobile phase,the column temperature was 30℃,and the flow rate was 1 mL/min.The results showed that under the chromatographic conditions,neotame had a good linear relationship with peak area in the mass concentration range of 0.2-10mg/L,the correlation coefficient was 0.999978,the limit of detection was 0.06 mg/kg,and the limit of quantification was 0.2mg/kg.The average recoveries of neotame spiked at the levels of 0.2,0.5 and 1.0 mg/kg were 97.1%-99.0%,and the relative standard deviation(n=6)was 0.39%-1.3%.The method was simple and rapid in pretreatment,which had good separation effect and low analysis cost in the whole inspection process,and provided a method for the rapid determination of neotame in liquor.