日粮中添加茉莉花渣对山羊营养物质表观消化率、瘤胃发酵及瘤胃菌群的影响

Effect of jasmine slag supplementation on apparent digestibility of nutrients,ruminal fermentation and rumen flora of goat

试验旨在探究日粮中添加茉莉花渣对山羊营养物质表观消化率、瘤胃发酵及瘤胃菌群的影响。选择健康无病、体重相近、4月龄的去势努比亚公羊24只,随机分为2组,每组12个重复,每个重复1只羊,对照组羊饲喂基础日粮,试验组在基础日粮中添加100 g/kg茉莉花渣,制成全价颗粒饲料。预试期7 d,正式试验期45 d。结果显示,与对照组相比,试验组山羊的粗蛋白表观消化率极显著降低(P<0.01),酸性洗涤纤维的表观消化率极显著提高(P<0.01)。微生物测序结果表明,对照组和试验组在门水平上的优势菌门均为厚壁菌门和拟杆菌门;试验组解琥珀酸菌属、普雷沃氏菌属_UCG_001和克里斯藤森菌科_R_7群相对丰度显著高于对照组(P<0.05),而未分类的拟杆菌科相对丰度显著低于对照组(P<0.05)。研究表明,日粮中添加茉莉花渣提高了纤维降解菌的相对丰度,改善了山羊对纤维物质的消化。

The experiment was to explore the effect of jasmine slag on apparent digestibility of nutrients,rumen fermentation and rumen bacterial flora of goats.Twenty-four healthy four-month-old Nubian rams with similar body weight and no disease were randomly divided into two groups,with 12 replicates in each group and one ram in each replicate.Rams in control group were fed with a basal diet,and those in experimental group were fed with a basal diet supplemented with 100 g/kg jasmine slag to make a full-price granular diet.The pre-test lasted for 7 d and the formal experiment lasted for 45 d.The results showed that compared with control group,the apparent digestibility of crude protein in experimental group was extremely decreased(P<0.01),and the apparent digestibility of acid detergent fiber was extremely increased(P<0.01).The results of microbial sequencing showed that Firmicutes and Bacteroides were the dominant phyla between control group and experimental group at phyla level.At genus level,the relative abundance of Succinobacteria,Prevotella_UCG_001 and Christiania_R_7 groups in experimental group was significantly higher than that in control group(P<0.05),while the relative abundance of unclassified Bacteroides in experimental group was significantly lower than that in control group(P<0.05).The experiment indicates that the addition of jasmine slag to the diet can increase the relative abundance of fiber degrading bacteria and improve the digestion of fiber in goats.