日粮添加鱼油对蛋鸡血清生化指标和蛋黄丙二醛含量的影响

Effect of dietary fish oil on serum biochemistry and yolk malondialdehyde content of laying hens

试验旨在研究在蛋鸡基础日粮中添加深海鱼油对蛋鸡血清生化指标、蛋黄丙二醛含量以及鸡蛋感官品质的影响。选取198日龄的海兰灰蛋鸡672只,随机分为6组,每组4个重复,每个重复28只蛋鸡。试验Ⅰ组(对照组)蛋鸡饲喂基础日粮,试验Ⅱ组、Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组分别在基础日粮中添加0.75%、1.00%、1.25%、1.50%、1.75%的深海鱼油。预试期7 d,正式试验期42 d。结果显示,与对照组相比,各试验组蛋鸡血清甘油三酯(TG)、胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量呈下降趋势,高密度脂蛋白胆固醇(HDL-C)含量有所上升。试验第7、14、42 d,各试验组蛋黄丙二醛(MDA)含量有所升高;试验第28 d,Ⅲ组和Ⅴ组蛋黄MDA含量分别比对照组降低21.51%和20.01%(P>0.05);随着试验时间增加,各试验组蛋黄MDA含量均有降低的趋势。与对照组相比,除Ⅵ组外,其余试验组鸡蛋的组织状态、口感、色泽以及整体感官评分增加,接受度提高,Ⅳ组得分最高,Ⅴ组其次,对Ⅵ组鸡蛋有轻微厌恶感。研究表明,蛋鸡基础日粮中的鱼油的适宜添加量为1.50%。

The experiment was to investigate the effect of supplementation of deep-sea fish oil in basic diet on serum biochemical indexes,malondialdehyde content in yolk and sensory quality of egg of laying hens.A total of six hundred and seventy-two 198-day-old Hyline laying hens were randomly divided into six groups,with four replicates in each group and 28 hens in each replicate.Laying hens in experimental groupⅠ(control group)were fed with basal diet,and laying hens in experimental groupⅡ,groupⅢ,groupⅣ,groupⅤand groupⅥwere fed with basal diet supplemented with 0.75%,1.00%,1.25%,1.50%and 1.75%deep-sea fish oil,respectively.The pre-test lasted for 7 d and the formal test lasted for 42 d.The results showed that compared with control group,the content of TG,TC and LDL-C of laying hens in experimental groups were decreased,while HDL-C was increased.On the 7th,14th and 42nd day,the content of MDA in egg yolk of experimental groups increased.On the 28th day of the experiment,MDA content in yolk of groupⅢand groupⅤdecreased by 21.51%and 20.01%compared with control group,respectively(P>0.05).With the increase of experiment time,the content of MDA in egg yolk of all experimental groups showed a decreasing trend.Compared with control group,except for groupⅥ,the tissue status,taste,color and overall sensory scores of eggs in experimental groups were increased,and the acceptability was improved,with the highest score of groupⅣ,followed by groupⅤ,which had a slight aversion to eggs in groupⅥ.The experiment indicates that the optimum supplemental amount of fish oil in the basic diet of laying hens is 1.50%.