结核分枝杆菌RNA恒温扩增实时荧光检测技术检测肺泡灌洗液诊断肺结核的价值

The diagnostic value of simultaneous amplification and testing for Mycobacterium tuberculosis technique on alveolar lavage fluid for diagnosing pulmonary tuberculosis

收集陕西省结核病防治院2021年10月至2022年7月疑似肺结核住院患者的肺泡灌洗液,同时进行涂片抗酸杆菌染色、分枝杆菌BACTEC MGIT 960液体培养(简称“培养”)及菌种鉴定、GeneXpert MTB/RIF、结核分枝杆菌RNA恒温扩增实时荧光检测技术(simultaneous amplification and testing for Mycobacterium tuberculosis,SAT-TB),并对结果进行分析。以培养法(菌种鉴定结果为结核分枝杆菌复合群)为参考标准,SAT-TB诊断肺结核的敏感度为80.61%(399/495),特异度为87.74%(1259/1435),阳性预测值为69.39%(399/575),阴性预测值为92.92%(1259/1355),约登指数为0.68。SAT-TB技术具有敏感度和特异度较高的特点,对临床快速诊断活动性结核病具有一定的价值。

Alveolar lavage fluid specimens from inpatients with clinical diagnosis of suspected tuberculosis in Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment from October 2021 to July 2022 were collected,and acid-fast bacilli smear,mycobacterium culture(MGIT 960)and strain identification,GeneXpert MTB/RIF and simultaneous amplification and testing for Mycobacterium tuberculosis(SAT-TB)test were performed.The results were analyzed and evaluated.Taking culture result(MGIT 960)as the standard,the sensitivity of SAT-TB in the diagnosis of tuberculosis was 80.61%(399/495),and the specificity was 87.74%(1259/1435),the positive predictive value was 69.39%(399/575),the negative predictive value was 92.92%(1259/1355),and the Youden index was 0.68.SAT-TB technique has high sensitivity and specificity,is of certain value for rapid diagnosis of active tuberculosis in clinic practice.