小球藻磁性碳中空微球作为药物缓释载体的开发和应用研究

Exploration and application of magnetic carbon hollow microsphere prepared from Chlorella sp. as medicine release carrier

目的 探究制备小球藻磁性碳中空微球的最佳条件及其装载诺氟沙星后的最佳控释条件。方法 采用水热碳化法合成了以Fe3O4为内核的磁性中空碳微球,并利用傅里叶红外光谱(FTIR)以及全自动比表面积和孔隙分析仪对微球进行表征测试。结果 FTIR结果显示微球的疏水性增加是藻壁纤维素亲水基团,如羧基、氨基均发生不同程度的减少造成的。通过全自动比表面积和孔隙度分析仪检测分析,有磁性碳化微球比表面积为0.596 m^(2)/g,微球孔径为3.054 nm。在对诺氟沙星的缓释研究中发现,在pH=7.0时220℃条件下合成的磁性碳中空微球每毫克可以装载诺氟沙星186.32μg,该磁性碳中空微球可以在pH=8.0的环境下实现最优的诺氟沙星控释,3.6 h的累积释药率可达到31%。结论 磁性碳中空微球在pH=8.0的环境下对药物的缓释作用使诺氟沙星可以在肠道中停留较长时间,对肠道中的致病菌起到应有的灭杀作用,并达到了一定程度的靶向给药目的。

Objective To explore the optimum conditions for the preparation of magnetic carbon hollow microspheres of Chlorella and the optimum controlled release conditions after loading norfloxacin. Methods The magnetic hollow carbon microspheres with Fe3O4as the core were synthesized through hydrothermal carbonization reaction, then Fourier transform infrared spectroscopy(FTIR) and automatic specific surface area and pore analyzer were used to characterize the microspheres. Results Through FTIR analysis, it was detected that the increase of hydrophobicity of the microspheres were caused by the reduction of functional groups carboxyl and amino groups. Through the detection and analysis of the automatic specific surface area and porosity analyzer, the specific surface area of the microspheres was 18.924 m^(2)/g and the pore diameter of the microspheres was 19.845 nm. The specific surface area of the magnetic carbonized microspheres was 0.596 m^(2)/g,and the pore size of the microspheres was 3.054 nm. In the sustained release study of norfloxacin, it was found that The magnetic carbon hollow microspheres synthesized at pH = 7.0 at 220 ℃ could contain 186.32 μg norfloxacin per mg, the magnetic carbon hollow microspheres could achieve the optimal controlled release process of norfloxacin in an environment of pH = 8.0 and the cumulative release rate of 3.6 hours could reach 31%. Conclusion the slow-release effect of magnetic carbon hollow microspheres on drugs in the environment of pH = 8.0 enables norfloxacin to stay in the intestinal tract for a long time, which could kill pathogenic bacteria in the intestinal tract and achieve targeted drug delivery to a certain extent.