基于双功能纳米探针的免疫层析技术在食品安全检测中的应用

Bifunctional nanoprobe-based immunochromatographic assays for food safety monitoring

免疫层析技术在基层食品安全监测中发挥着举足轻重的作用。探针材料是决定免疫层析试纸条检测性能的关键。大量研究表明,基于功能集成化策略的双功能纳米材料作为探针可极大地提升免疫层析方法的性能。基于双信号探针的免疫层析方法、基于磁性光学纳米探针的免疫层析方法和基于探针催化信号放大的多级比色免疫层析方法因优异的分析性能和巨大的应用潜力而备受关注。该文详细阐述了这三类免疫层析技术的原理、发展现状及其在食品安全检测方面的应用,并讨论了该领域的发展趋势和面临的主要挑战。

Immunochromatographic assay(ICA)plays a crucial role in daily food safety inspection.Materials for probes are key factors to determine the analytical performances of ICA test strips.Numerous studies have demonstrated that bifunctional probes based on functional integration can significantly improve the performance of ICA.Dual-signal probe-,magnetic-optical nanoprobe-based ICAs and multilevel colorimetric ICA reinforced by signal amplification generated by catalytic probes have attracted much attention due to their manifested analytical properties and application potential.Herein,we review the principles of the above three types of ICAs,their recent advances,and applications for food safety.We also discuss the challenges and future prospects of this field.