非洲绿猴TRIM3基因的克隆与序列分析

Cloning and sequence analysis of TRIM3 gene in African green monkey

对非洲绿猴三基序结合蛋白3的基因(TRIM3基因)进行克隆并进行序列分析。扩增非洲绿猴TRIM3基因编码区域(CDS),应用在线软件进行生物信息学分析。非洲绿猴TRIM3基因CDS序列全长2235 bp,编码744个氨基酸。核苷酸相似性分析结果显示,非洲绿猴与猕猴相似性最高,相似性为99.5%,与石龟的相似性为75.3%。生物信息学分析显示,非洲绿猴三基序结合蛋白3分子质量为80.93 ku,理论等电点为8.03,不稳定系数为40.01,是不稳定蛋白。亲水性分析显示,三基序结合蛋白3为亲水性蛋白,不是分泌蛋白,预测该蛋白有22个磷酸化位点。三基序结合蛋白3二级结构以延伸链为主,占38.84%。

The purpose of the experiment was to clone and sequence gene of the African green monkey trimotif binding protein 3 gene(TRIM3).The TRIM3 gene coding region(CDS)of African green monkey was amplified,and bioinformatics analysis was carried out using a variety of online software.The CDS sequence of TRIM3 gene of African green monkey was 2235 bp in length,encoding 744 amino acids.The results of nucleotide similarity analysis showed that the similarity between African green monkey and macaque was up to 99.5%,and the similarity between African green monkey and stone turtle was 75.3%.Bioinformatics analysis shows that the molecular weight of trimotif binding protein 3 of African green monkey was 80.93 ku,the theoretical isoelectric point is 8.03,and the instability coefficient is 40.01,which is an unstable protein.The hydrophilicity analysis showed that trimotif binding protein 3 was a hydrophilic protein,not a secretory protein.It was predicted that the protein had 22 phosphorylation sites.The secondary structure of trimotif binding protein 3 protein is mainly extended chain,accounting for 38.84%.