一种新型分离传感膜的制备及其血糖的动态监测

Preparation of a novel separation-sensing membrane and its dynamic monitoring of blood glucose

血糖是临床手术中非常重要的生理指标之一,其不仅能灵敏反映术中患者的肾脏功能情况,还直接影响了术后愈合及生理康复。然而,现有的临床检测设备,如血糖仪、生化分析仪等,仅能间歇性反馈检测结果,难以实现术中病患血糖的动态监测。针对以上难题,提出将膜分离技术与生物传感技术相结合,构建可同步实现全血动态分离与血糖在线监测的分离传感膜。通过层层自组装法将普鲁士蓝(PB)纳米颗粒和金(Au)纳米颗粒原位共生长在钇稳定的氧化锆(YSZ)陶瓷中空纤维膜上,探究了不同组装层数的PB/Au对分离传感膜的表面亲疏水性的影响,评估最佳制膜条件下的血浆分离效果与血糖电化学检测性能。结果表明,当PB/Au组装层数为60层时所得分离传感膜具有最佳的亲水性,且能完全截留住全血中的红细胞、白细胞及血小板。该分离传感膜对葡萄糖的检测灵敏度为0.876μA/(mmol/L),检测范围达到1~15 mmol/L,并能同步实现真实人血样本的动态分离与检测过程。

Blood glucose is one of the most important physiological indicators.In clinical surgery,blood glucose can sensitively reflect the kidney function of patients to affect the postoperative healing and physiological.However,the existing clinical testing equipment,such as blood glucose meters,biochemical analyzers,etc.,can only provide intermittent feedback of test results,and it is difficult to achieve dynamic monitoring of patients’blood sugar during surgery.As they can only collect intermittent feedback of detection results rely on discrete index points.In view of the above problems,this work proposed the combination of membrane separation technology and biosensing technology to construct a separation-sensing membrane which can synchronously realize dynamic separation of whole blood and online monitoring of blood glucose.Prussian blue(PB)nanoparticles and gold(Au)nanoparticles were grown in situ and synchronously on zirconia ceramic hollow fiber membrane(YSZ)via layer-by-layer selfassembly method.The effect of PB/Au on the hydrophilicity and hydrophobicity of the sensing membrane was investigated,and the plasma separation effect and blood glucose electrochemical detection performance were evaluated under the optimal membrane preparation conditions.The results showed that the best condition was 60 layers which can completely reject all the red blood cells,white blood cells and platelets in the whole blood.The separation-sensing membrane showed a sensitivity of 0.876μA/(mmol/L),a liner range of 1—15 mmol/L to blood glucose and realized dynamic separation and detection in real human blood samples.