涠洲岛火山口真菌的分离及次级代谢产物鉴定

Fungi from Weizhou Island crater and identification of their secondary metabolites

涠洲岛火山口生态环境特殊,蕴藏着丰富且独具特色的微生物资源。关于涠洲岛火山口海洋真菌来源的次级代谢产物鲜有报道。本研究采用两种培养基从涠洲岛火山口海洋植物中分离真菌,对菌株的代谢产物进行分离纯化,并通过波谱等方法鉴定化合物结构。从4种涠洲岛火山口附近的海洋植物样本中分离真菌共31株,从菌株青霉菌Penicillium sp.TX-M1-3和Penicillium sp.LW-2-1的发酵物中纯化获得2个主要次级代谢产物,并鉴定为核丛青霉素和弯孢霉菌素。活性评价表明核丛青霉素对NPC1L1蛋白具有一定的抑制作用,暗示其在治疗心血管疾病方面的潜力。本结果拓展了涠洲岛火山口微生物资源及其次生代谢产物方面的研究,为该地区微生物与其次生代谢产物的研究提供了相关基础。

Weizhou Island crater as a special ecological environment,contains rich and diverse fungal resources,and there are few reports on the fungi and their secondary metabolites of the crater area.In this study,two kinds of media were used to isolate fungi from marine plants in Weizhou Island crater,and different approaches were also used to purify and elucidate secondary metabolites from the isolated strains.In total,31 fungal strains were isolated.Sclerotiorin and curvularin were identified as the main secondary metabolites from Penicillium sp.TX-M1-3 and Penicillium sp.LW-2-1,respectively.This study reports the inhibitory effect of sclerotiorin on NPC1L1 protein for the first time,proving its potential in the treatment of cardiovascular disease.This is the first report about microbial resources and their secondary metabolites in Weizhou Island crater,providing a relevant basis for further study of fungal diversity and utilization of the resources in this area.