响应面法优化解脂亚罗酵母产菜油甾醇培养基组成

Optimization of medium composition for campesterol production in Yarrowia lipolytica by response surface methodology

为提高解脂亚罗酵母PMEDD菌株菜油甾醇的产量,对此菌株发酵培养基组成进行了优化。采用单因素实验探究碳源种类、氮源种类、碳源质量浓度、氮源质量浓度、KH2PO4质量浓度对菜油甾醇产量的影响。在此基础上,采用响应面实验对发酵培养基组成进行了优化。结果表明:培养基组成为葡萄糖3.17 g/100 mL,蛋白胨1.44 g/100 mL,KH2PO40.65 g/100 mL,Uracil 0.1 g/100 mL,YNB 0.42 g/100 mL,MgSO4·7H2O 0.15 g/100 mL时,菜油甾醇的产量达到最大,为16.49μg/mL,优化后菜油甾醇的产量为优化前的2.10倍。采用优化后的培养基,可有效提高解脂亚罗酵母PMEDD菌株菜油甾醇的产量。

In order to increase the yield of campesterol in Yarrowia lipolytica PMEDD, the fermentation medium composition was optimized. The influences of carbon source type, nitrogen source type, carbon source mass concentration, nitrogen source mass concentration, and KH2PO4mass concentration on the yield of campesterol were explored by single factor experiment. Then the fermentation medium composition was optimized by response surface methodology. The results showed that the optimal medium composition was obained as follows: glucose 3.17 g/100 mL, peptone 1.44 g/100 mL, KH2PO40.65 g/100 mL, Uracil 0.1 g/100 mL, YNB 0.42 g/100 mL, MgSO4·7H2O 0.15 g/100 mL. Under these conditions, the maximal campesterol production of 16.49 μg/mL was obtained, which was 2.10 times of the initial fermentation medium. The optimized medium can effectively increase the yield of campesterol in Yarrowia lipolytica PMEDD.