长链非编码RNA01089-209在乳腺癌患者血清 外泌体中的表达及与临床病理特征的关系

Expression of long non-coding RNA01089-209 in breast cancer and its effect on clinicopathology

目的了解乳腺癌及乳腺良性肿瘤血清外泌体中lncRNA的表达情况、乳腺癌患者血清外泌体中LncRNA01089-209的表达水平与临床病理特征之间的关系。方法收集乳腺癌患者及乳腺良性肿瘤患者血清外泌体各4例,进行测序、验证及生物信息学分析筛选差异表达lncRNA,对差异lncRNA进行靶基因预测与富集分析,确定目标lncRNA,进一步研究乳腺癌发生、发展的机制。采用RT-PCR技术验证在乳腺癌患者血清外泌体中LncRNA01089-209的表达情况,以及与其临床病理特征的关系。结果在4对乳腺浸润性导管癌与乳腺良性肿瘤的LncRNA-Seq测序检测结果显示:LncRNA01089-209在乳腺癌患者血清外泌体中显著低表达(P=0.025),并可能在自然杀伤细胞介导的细胞毒性通路中起重要的作用。RT-PCR检测结果及临床病理结果分析显示:乳腺癌血清外泌体中LncRNA01089-209表达水平与乳腺癌患者患病年龄、绝经状况、ER表达情况、淋巴结转移与分子分型相关(均P<0.05)。结论LncRNA01089-209在LuminalB型乳腺癌血清外泌体中显著低表达,与ER表达情况、淋巴结转移、患病年龄和绝经状况等显著相关,可作为乳腺癌患者判断预后的潜在生物学标志物和治疗靶点。

ObjectiveTo investigate the expression of lncRNA in exocrine bodies of breast cancer and benign breast tumors,and the relationship between the expression of LncRNA01089-209 in exocrine bodies of patients with breast cancer and clinicopathological features.MethodsFour patients with breast cancer and four patients with benign breast tumor were collected.The differential expression of lncRNA was analyzed by sequencing,platform alignment and bioinformatics analysis.The target gene prediction and enrichment analysis of differential lncRNA were performed to find the target lncRNA.RT-PCR technique was used to verify the expression level of LncRNA01089-209 in breast cancer,and the correlation between theexpression level of LncRNA01089-209 inbreast cancer and clinicopathological parameters was analyzed.ResultsThe LncRNA-Seq sequencing detection results about four pairs with invesive breast cancer and bengin breast tumer:The expression of LncRNA01089-209 in exocrine of breast cancer patients was significantly low(P=0.025).It maybe related toan important role in natural killer cytotoxicity pathway.RT-PCR and clinicopathological analysis showed that the expression level of LncRNA01089-209 in serum exosomes of breast cancer was correlated with age,menopause,ER expression,lymph node metastasis and molecular typing of breast cancer patients(all P<0.05).ConclusionThe expression of LncRNA01089-209 in exosomes of LuminalB breast cancer is significantly low,which is significantly related to the expression of ER,lymph node metastasis and menstrual status.It can be used as apotential biomaker for clinical diagnosis and treatment of breast cancer.