基于高通量测序分析高粱紫斑病叶片的微生物组成及多样性

Analysis of Microbial Composition and Diversity of Sorghum Purple Spot Leaves based on High-throughput Sequencing

本研究采用ITS高通量测序(扩增子测序)技术对已感染紫斑病(A和B)和未感染紫斑病(CK)的高粱叶片进行检测,以明确其真菌的群落组成及多样性。结果表明:高粱紫斑病病叶中真菌菌群组成丰富,总共检出820种真菌,3个样品共同拥有的为155种,所有菌群分属于9门29纲60目122科和206属。3份样品Shannon指数分别为3.98、4.67、4.11,按大小排序为B>CK>A。同时,含量排名靠前的属之间亲缘关系比较复杂,且没有出现前人报道的高粱尾孢Cercospora sorghi,可能的原因是引起该病害发生的病原菌发生了变化,或是存在由多种真菌共同作用从而导致病害发生的可能性。本研究结果能帮助我们更好地了解高粱紫斑病发病期叶片真菌的组成和分布的多样性,为该病害的研究和防治提供更好的指导作用。

ITS high-throughput sequencing technology was used to detect sorghum leaves infected and uninfested with purple spot disease, to determine the composition and diversity of fungal community.The results showed that a total of 820 species OTUs were obtained from fungal community in sorghum purple leaf spot, 155 OTU species were shared by the three samples.All these species belonged to 9 phylum, 29 classes, 60 orders, 122 families and 206 genus.The Shannon index relation of the three samples was 3.98,4.67 and 4.11,respectively, which were in the order of B>CK>A.Genetic relationship among the top ten genus were relatively complex, and Cercospora sorghi reported by previous reports was not included in them.We presumed that the pathogen changed or was caused by the combined action of multiple fungi.The results of this study could help us better understand the composition and distribution diversity of leaf fungi during the onset of sorghum purple spot disease.It has a better guiding function for the research and control of this disease.