南极磷虾油对2型糖尿病小鼠降血糖功效及其机制研究

Hypoglycemic Effect of Antarctic Krill Oil on Type 2 Diabetes Mellitus Mice and the Mechanism

探究南极磷虾油的降血糖功效及相关机制。以雄性4周龄C57BL/6J小鼠为研究对象,除空白对照组(BCG)以外,以高脂高糖饲料结合链脲佐菌素的方法建立糖尿病模型,然后将小鼠分为低剂量南极磷虾油组(LDG,100 mg/kg)、中剂量南极磷虾油组(MDG,250 mg/kg)、高剂量南极磷虾油组(HDG,500 mg/kg)、糖尿病模型组(NCG)和阳性对照组(PCG),LDG、MDG、HDG分别灌胃不同剂量的南极磷虾油,BCG、NCG两组灌胃生理盐水,PCG灌胃二甲双胍。灌胃期间,监测小鼠空腹血糖(fasting blood glucose,FBG)值及体质量变化趋势,灌胃结束后,对小鼠进行口服葡萄糖耐量实验(oral glucose tolerance test,OGTT),检测糖化血红蛋白(glycosylated hemoglobin type A1c,HbA1c)水平、空腹胰岛素(fasting insulin,FINS)水平以及抗氧化能力和抗炎能力等相关指标。结果表明:灌胃南极磷虾油的3个剂量组较糖尿病模型组,FBG值、HbA1c水平、AUC均有所改善,FINS分泌量均显著提高(p<0.05),白细胞介素-1、白细胞介素-6、肿瘤坏死因子的mRNA相对表达量显著减少(p<0.05);HDG、MDG两组的超氧化物歧化酶活性显著高于糖尿病模型组(p<0.05);HDG的过氧化氢酶活性、谷胱甘肽过氧化物酶活性显著高于糖尿病模型组(p<0.05),丙二醛含量显著低于糖尿病模型组(p<0.05)。南极磷虾油可以改善小鼠抗氧化能力和抗炎能力,从而达到缓解糖尿病的作用。

This study aims to investigate the hypoglycemic efficacy of Antarctic krill oil and the mechanism.The 4-week-old male C57BL/6J mice were used.The high-fat high-sugar feed and streptozotocin were used to induce type 2 diabetes mellitus in mice except for the blank control group(BCG,normal saline),and the model mice were classified into low-dose Antarctic krill oil group(LDG:100 mg/kg,ig),medium-dose Antarctic krill oil group(MDG:250 mg/kg),high-dose Antarctic krill oil group(HDG:500 mg/kg),negative control group(NCG,normal saline),and positive control group(PCG,metformin).During the gavage(8 weeks),the fasting blood glucose(FBG)and body weight of mice were monitored each week.After the gavage,the oral glucose tolerance test(OGTT)was conducted and glycosylated hemoglobin type A1c(HbA1c)content,fasting insulin(FINS)content,antioxidant ability [superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)],and the inflammatory cytokines were determined.The result showed that HDG,MDG,and LDG had low levels of FBG,HbA1c,AUC,high FINS content(p0.05),and low relative mRNA expression of interleukin-1(IL-1),interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α)(p0.05)compared with the NCG.SOD activity in HDG and MDG was significantly higher than that in NCG(p0.05).HDG had significantly higher CAT activity and GSH-Px activity(p 0.05),lower MDA content(p 0.05),and significantly lower TNF-α relative mRNA expression(p 0.05) than the NCG.In conclusion,Antarctic krill oil can alleviate diabetes by improving the antioxidant and anti-inflammatory abilities of mice.