新型前列腺尿道悬吊术植入物的生物相容性

Biocompatibility of a new prostatic urethral lift implant

目的探讨新型前列腺尿道悬吊术(PUL)植入物的生物相容性。方法人前列腺增生细胞系(BPH-1)分别培养于植入物第一锚钉、第二锚钉及缝线浸提液作为实验组,细胞培养于完全培养基作为对照组,培养1、3、5 d,细胞计数试剂盒(CCK-8)检测细胞活力,评估植入物细胞毒性;鬼笔环肽染色,评估植入物对细胞骨架的影响。BPH-1细胞与第一锚钉、第二锚钉共培养5 d,于扫描电子显微镜(SEM)下观察前列腺细胞在植入物上的黏附状态。Sprague Dawley(SD)大鼠20只(8~10周龄,体重210~250 g),购自湖北省疾病预防控制中心,大鼠背部肌肉分别植入第一锚钉、第二锚钉及缝线,对照组行假手术仅分离肌肉,观察、监测12周大鼠临床反应及体重变化,评估植入物全身毒性。多组间比较采用单因素方差分析。结果CCK-8细胞毒性实验显示,第一锚钉组第1、3、5天时BPH-1细胞相对存活率分别为94.49%、95.91%、97.55%;第二锚钉组分别为94.76%、95.16%、98.37%;缝线组分别为95.24%、96.99%、98.74%。SEM结果显示,BPH-1细胞在第一锚钉及第二锚钉上正常黏附、生长;鬼笔环肽染色显示,第一锚钉组、第二锚钉组及缝线组细胞骨架阳性表达面积分别为(20.85±3.06)%、(21.06±2.54)%、(21.27±2.63)%与对照组[(21.40±2.21)%]比较差异无统计学意义(F=0.041,P>0.05),植入物对细胞骨架分布无影响。全身毒性实验结果显示,大鼠一般状态良好,无异常临床反应,对照组,第一锚钉组、第二锚钉组及缝线组术后12周体重分别为(574.2±31.1)、(567.8±41.6)、(569.2±34.0)、(571.0±31.6)g,组间差异无统计学意义(F=0.0314,P>0.05),植入物无大鼠全身毒性作用。结论新型PUL植入物是一种无毒性,与前列腺细胞相容性好的材料。

Objective To investigate the biocompatibility of a new prostatic urethral lift(PUL)implant.Methods Human prostatic hyperplasia cell line(BPH-1)was cultured at the first,second anchor and suture extract of the implant as the experimental group,while the cells were cultured in complete culture medium as the control group for 1 day,3 days,5 days.Cell counting kit-8(CCK-8)assay was used to detect cell viability and evaluate implant cytotoxicity.Ghost pen cyclic peptide staining was used to evaluate the effect of implants on the cytoskeleton.BPH-1 cells were co-cultured with the first anchor and the second anchor for 5 days,and the adhesion status of prostate cells on the implant was observed under a scanning electron microscope(SEM).A total of 20 Sprague Dawley(SD)rats(8-10 weeks old,weighing 210-250 g)were obtained from Hubei Center for Disease Control and Prevention.The back muscles of the rats were implanted with the first anchor,the second anchor and suture,respectively.Sham operation was performed on the control group to separate the muscles.One-way ANOVA analysis was used to compare groups.Results CCK-8 assay showed that the relative survival rates of BPH-1 cells on day 1,day 3 and day 5 in the first anchor group were 94.49%,95.91% and 97.55%,respectively.In the second anchor group,the results were 94.76%,95.16% and 98.37%,respectively.In the sutures group,the results were 95.24%,96.99% and 98.74%,respectively,without cytotoxicity.The SEM results showed that BPH-1 cells adhered and grew normally on the first and second anchors.Ghost pen cyclic peptide staining showed that the cytoskeleton positive expression area(%)of the first anchor group,the second anchor group and the suture group was 20.85±3.06,21.06±2.54,21.27±2.63,which showed no statistical significance from the control group(21.40±2.21,F=0.041,P>0.05).Systemic toxicity test results showed that the rats were generally in good condition without abnormal clinical reactions.The body weight(g)of the control group,the first anchor group,the second anchor group and the suture group at 12th week after surgery was as follows:574.2±31.1,567.8±41.6,569.2±34.0,571.0±31.6,without statistical significance among the groups(F=0.0314,P>0.05).The implant had no systemic toxicity in rats.Conclusion The new PUL implant is a non-toxic material with good compatibility with prostate cells.