生物钟蛋白Rev-erbα在不同程度的前列腺组织炎症中的表达变化

Expression of circadian clock protein Rev-erbα in prostate tissues of different degrees of inflammation

目的:探讨良性前列腺增生(BPH)人群中前列腺组织炎症与生物钟蛋白Rev-erbα表达的相关性。方法:收集诊断为BPH并行经尿道前列腺手术的前列腺组织标本。对前列腺组织进行炎症程度分级。免疫组化(IHC)评估前列腺组织中CD4、CD8、白细胞介素(IL)-6、IL-8、IL-1β和Rev-erbα的表达情况;qRT-PCR检测前列腺组织中IL-1β、IL-6和IL-8和Rev-erbα的表达水平。分析前列腺组织炎症程度与前列腺组织Rev-erbα表达强度的相关性。结果:收集84例BPH组织标本,73例(86.9%)伴有不同程度的组织炎症。IHC提示前列腺组织中不同炎症程度与炎症因子(IL-6、IL-8和IL-1β)表达强度一致;组织炎症细胞中,CD4^(+)细胞数量远多于CD8^(+)细胞数量;前列腺组织中Rev-erbα的表达强度与前列腺组织炎症程度呈负相关,其相关系数为-0.691(P<0.001)。qRT-PCR提示不同的前列腺组织炎症程度与组织中IL-1β、IL-6、IL-8的mRNA表达差异趋势一致,与组织中Rev-erbα的mRNA表达差异趋势相反。结论:生物钟蛋白Rev-erbα可能在前列腺组织中有降低组织局部炎症的作用,可能成为防治前列腺组织炎症的潜在靶点。

Objective:To investigate the relationship between inflammation of prostate tissue and the expression of clock protein Rev-erbα in prostatic tissues.Methods:Prostate tissue specimens diagnosed as BPH and undergoing transurethral prostate surgery were collected.The inflammation degree of prostate tissue was graded.Immunohistochemistry(IHC) was used to evaluate the expression of CD4,CD8,IL-6,IL-8,IL-1β and Rev-erbα in prostate tissue,and the expression level of IL-1β,IL-6,IL-8 and Rev-erbα were detected by qRT-PCR in prostate tissues.The correlation between the degree of inflammation in prostate tissue and the expression intensity of Rev-erbα in prostate tissue was analyzed.Results:A total of 84 cases of prostate tissue specimens were collected,of which 73 cases(86.9%) were accompanied by tissue inflammation.IHC showed that different levels of inflammation in prostate tissue were consistent with the expression intensity of inflammatory factors(IL-6,IL-8 and IL-1β),and the number of CD4~+ cells was far greater than the number of CD8~+ cells.The expression intensity of Rev-erbα in prostate tissue was negatively correlated with the degree inflammation in prostate tissues,and the correlation coefficient was-0.691(P0.001).QRT-PCR indicated the trend of differences in the expression of IL-1β,IL-6 and IL-8 was consistent with the trend of the degree of inflammation in prostate tissues,but the trend of differences in the expression of Rev-erbα was opposite to the trend of the degree of inflammation in prostate tissues.Conclusion:The circadian clock protein Rev-erbα may have the effect of reducing local inflammation in the prostate tissue,and it may become a potential target for the prevention and treatment of inflammation in the prostate tissue.