DDIT3免疫组织化学抗体在53例黏液样脂肪肉瘤中的表达及诊断价值

Expression and diagnostic value of DDIT3 immunohistochemical antibody in 53 cases of myxoid liposarcoma

目的:探讨DDIT3免疫组织化学抗体在黏液样脂肪肉瘤中的诊断价值。方法:收集北京积水潭医院2010—2022年诊断为黏液样脂肪肉瘤且荧光原位杂交(FISH)检测具有DDIT3基因重排的病例53例为病例组,收集同时期组织形态需与黏液样脂肪肉瘤相鉴别的其他肿瘤50例作为对照组。通过免疫组织化学检测DDIT3抗体在黏液样脂肪肉瘤中的表达情况,对比对照组病例中的表达情况,分析DDIT3免疫组织化学抗体在黏液样脂肪肉瘤诊断中的特异度及灵敏度,同时分析DDIT3免疫组织化学抗体表达情况与FISH检测DDIT3基因重排结果的相关性。结果:53例黏液样脂肪肉瘤病例中,男性31例,女性22例,患者年龄11~74岁,中位年龄51岁,平均年龄49.4岁。DDIT3免疫组织化学抗体在53例黏液样脂肪肉瘤中的表达率为100%,阳性细胞比例为30%~100%不等,强度为弱阳至强阳不等。在对照组病例中未见阳性表达,极少数对照组病例中见散在个别细胞表达(<10%)。DDIT3免疫组织化学表达阳性细胞比例及强度与FISH检测的DDIT3基因重排的阳性细胞比例及DDIT3重排伙伴基因未见显著相关性。结论:DDIT3免疫组织化学抗体在黏液样脂肪肉瘤诊断中的灵敏度及特异度均达到100%,与FISH检测方法结果一致,可作为方便、经济的黏液样脂肪肉瘤诊断指标推广应用。

Objective To investigate the diagnostic value of DDIT3 immunohistochemical antibody in myxoid liposarcoma.Methods A total of 53 patients diagnosed with myxoid liposarcoma and with DDIT3 gene rearrangement detected by fluorescence in situ hybridization(FISH)from 2010 to 2022 in Beijing Jishuitan Hospital were enrolled as the case group,and 50 patients with other tumors that needed to be differentiated from myxoid liposarcoma during the same period were enrolled as the control group.The immunochemical(IHC)expression of DDIT3 was evaluated in all cases and the specificity and sensitivity in the diagnosis of myxoid liposarcoma were calculated.The correlation between IHC DDIT3 expression and FISH detection of DDIT3 gene rearrangement was analyzed.Results Among the 53 cases of myxoid liposarcoma,thirty-one patients were males and twenty-two were females.The patients ranged in age from 11 to 74 years,with a median age of 51 and an average age of 49.4 years.The expression rate of IHC DDIT3 expression in 53 cases of myxoid liposarcoma was 100%.No expression was found or only scattered individual cell(<10%)expression was seen in a very few cases in the.control group.There was no significant correlation between the proportion and intensity of IHC and FISH DDIT3 positive cells or the partner gene.Conclusions The sensitivity and specificity of DDIT3 immunohistochemical antibody in the diagnosis of myxoid liposarcoma are both 100%,which are consistent with the results of FISH.It can be used as a convenient and economical diagnostic tool for myxoid liposarcoma.