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新西兰白兔胃左及胃十二指肠动脉栓塞减肥术后组织病理及免疫组织化学变化

Histopathological and immunohistochemical changes of gastric fundus and duodenum after bariatric embolization in the New Zealand rabbits
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摘要 目的探究新西兰白兔肥胖模型经导管胃左动脉及胃十二指肠动脉栓塞术后胃及十二指肠组织病理及免疫组织化学变化。方法成功建立新西兰白兔肥胖模型20只, 分层随机分成2组, 实验组经导管明胶海绵颗粒(350~560 μm)栓塞新西兰兔胃左动脉及胃十二指肠动脉, 对照组经导管生理盐水(5 ml)灌注胃左动脉及胃十二指肠动脉, 术后4周处死动物行胃及十二指肠组织病理、免疫组织化学检查及Western Blot检测, 测量产胃饥饿素细胞密度及胃饥饿素蛋白条带灰度比值, 采用独立样本t检验进行分析比较。结果大体病理检查提示1只实验组新西兰白兔胃体部前壁出现溃疡, 组织病理学检查提示3只实验组新西兰白兔胃体部黏膜溃疡, 免疫组织化学检查提示实验组胃基底部及十二指肠产胃饥饿素细胞较对照组显著减少(胃基底部分别为10.0±5.1、27.7±3.4, t=12.35, P<0.05;十二指肠分别为5.6±2.6、12.3±2.1, t=4.73, P<0.05)。Western Blot检测提示实验组胃基底部及十二指肠胃饥饿素蛋白条带灰度比值较对照组显著降低(胃基底部分别为0.65±0.05、1.12±0.09, t=9.62, P<0.05;十二指肠分别为0.55±0.03、0.94±0.08, t=7.98, P<0.05)。结论胃左及十二指肠动脉栓塞减肥术后免疫组织化学及Western Blot检测证实实验组白兔胃基底部及十二指肠的产胃饥饿素细胞较对照组明显减少, 且术后组织病理学检查提示此技术较为安全。 Objective To investigate the histopathological and immunohistochemical changes of gastric fundus and duodenum after bariatric embolization of left gastric artery and gastroduodenal artery in obesity New Zealand rabbit models.Methods Twenty obesity New Zealand rabbit models were successfully established and divided into two groups using stratified randomization.Left gastric artery and gastroduodenal artery were embolized with gelatin sponge(350-560μm)in experimental group,left gastric artery and gastroduodenal artery were perfused with normal saline in control group.All animals were sacrificed for pathological,immunohistochemical examination and Western Blot analysis 4 weeks post embolization,the density of ghrelin producing cells and the gray ratio of ghrelin protein band were measured and compared by the independent sample t test.Results Macropathological examination showed ulceration in the anterior wall of the gastric body in one rabbit,histopathological examination showed mucosa ulceration in the gastric body in 3 rabbits in experimental group.Immunohistochemical examination showed that the ghrelin producing cells of gastric fundus and duodenum in the experimental group were significantly less than those in the control group(10.0±5.1 vs.27.7±3.4,t=12.35,P<0.05;5.6±2.6 vs.12.3±2.1,t=4.73,P<0.05).Western Blot analysis showed that the gray ratio of ghrelin bands of gastric fundus and duodenum in the experimental group were significantly lower than that in the control group(0.65±0.05 vs.1.12±0.09,t=9.62,P<0.05;0.55±0.03 vs.0.94±0.08,t=7.98,P<0.05).Conclusions Immunohistochemical and Western Blot analysis showed that the ghrelin-producing cells of gastric fundus and duodenum in the experimental group were significantly less than those in the control group after bariatric embolization,histopathologic analysis indicated that bariatric embolization was a safe technique.
作者 付金鑫 段峰 张金龙 袁冰 张恒 阎洁羽 管阳 王燕 袁凯 王茂强 Jinxin Fu;Feng Duan;Jinlong Zhang;Bing Yuan;Heng Zhang;Jieyu Yan;Yang Guan;Yan Wang;Kai Yuan;Maoqiang Wang(Department of Interventional Radiology,the Fifth Medical Center of PLA General Hospital,Beijing 100039,China;Department of Radiology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China)
出处 《中华放射学杂志》 CAS CSCD 北大核心 2023年第3期306-310,共5页 Chinese Journal of Radiology
关键词 放射学 介入性 胃左动脉栓塞 组织病理 免疫组织化学 胃饥饿素 Radiology,interventional Left gastric artery embolization Histopathology Immunohistochemistry Ghrelin
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