摘要
试验旨在优化超声辅助双水相法提取辛夷总黄酮的工艺条件,研究其体外抗氧化活性。以总黄酮得率为指标,采用单因素和响应面试验设计优化最佳提取条件,DPPH自由基清除率初步评价辛夷总黄酮的抗氧化活性。结果显示,超声辅助双水相法提取辛夷总黄酮最佳提取条件为聚乙二醇(PEG1000)质量分数40%、硫酸铵质量分数30%、液料比40 mL/g、提取时间30 min;此条件下对建立的数学模型进行试验验证,验证结果预测值为6.78%,实际值为6.81%,预测误差为0.79%。辛夷总黄酮对DPPH自由基清除率达77.36%,具有良好的抗氧化活性。研究表明,超声辅助双水相法操作简便,黄酮得率高,适用于辛夷总黄酮的提取。
The study was to optimize the extraction conditions and antioxidant activity of total flavonoids from Magnolia biondii Pamp. by ultrasonic-assisted two-phase aqueous method. Single factor and response surface design were used to optimize the optimal extraction conditions, and DPPH free radical scavenging rate was used to evaluate the antioxidant activity of total flavonoids of Magnolia biondii Pamp. The results showed that the optimal extraction conditions were PEG1000 mass fraction 40%, ammonium sulfate mass fraction 30%, liquid to soild ratio 40 mL/g and extraction time 30 min. Under the condition, the mathematical model was verified by experiment, and the verification result showed that the predicted value was 6.78%, the actual value was 6.81%, and the prediction error was 0.79%. The DPPH free radical scavenging rate of total flavonoids of Magnolia biondii Pamp. was 77.36%, which had good antioxidant activity. The study indicates that the ultrasonic-assisted two-water phase method is simple and suitable for the extraction of flavonoids from Magnolia biondii Pamp.
作者
高利
岳璐
杨增航
耿紫馨
秦晶晶
GAO Li;YUE Lu;YANG Zeng-hang;GENG Zi-xin;QIN Jing-jing
出处
《饲料研究》
CAS
北大核心
2023年第4期90-94,共5页
Feed Research
基金
新乡市科技攻关(项目编号:GG2021021)
河南省科技攻关项目(项目编号:212102110187)
新乡医学院三全学院骨干教师培养计划(项目编号:SQ2021GGJS02)
新乡医学院三全学院科学研究培育基金项目(项目编号:GG2021021XJKT201921、XJKT201919)。
关键词
辛夷
总黄酮
双水相提取
响应面分析
抗氧化活性
Magnolia biondii Pamp.
total flavonoids
two aqueous phase extraction
response surface analysis
antioxidant activity