旋覆花-赭石药对不同配伍比例单煎与合煎化学成分对比分析

Contrast analysis of chemical constituents between single decoction and mixed decoction of Inulae Flos-Haematitum medicinal pair with different proportion

目的比较旋覆花-赭石药对不同配伍比例单煎、合煎化学成分的差异性。方法采用超高效液相色谱(UPLC)法测定4组不同配伍比例旋覆花-赭石药对单煎与合煎样品中新绿原酸、绿原酸、隐绿原酸、咖啡酸、异槲皮苷、异绿原酸B、1,5-二咖啡酰奎宁酸、异绿原酸C 8个指标成分的含量,并采集各样品的指纹图谱,计算指纹图谱中各共有峰的“峰面积/称样量”值,采用SPSS 26.0进行独立样本t检验分析。结果指纹图谱中共标定了16个特征峰,不同配伍比例旋覆花-赭石药对单煎与合煎样品中,峰1、峰2、峰4、峰6、峰9、峰10、峰12、峰13、峰15的“峰面积/称样量”值比较,差异有统计学意义(P<0.05);当旋覆花、赭石配伍比例为3∶1时,单煎与合煎样品指纹图谱及指标成分含量差异较大,除峰7、峰14外,其余特征峰的“峰面积/称样量”值比较,差异有统计学意义(P<0.05),且8个指标成分含量比较,差异有统计学意义(P<0.05)。结论不同配伍比例旋覆花-赭石药对单煎与合煎的化学成分存在差异。

Objective To compare the differences of chemical components between single decoction and mixed decoction with different compatibility ratio of Inulae Flos-Haematitum medicinal pair.Methods UPLC method was used to determine the contents of 5-caffeoylquinic acid,chlorogenic acid,4-dicaffeoylquinic acid,caffeic acid,isoquercitrin,isochlorogenic acid B,1,5-dicaffeoyl quinic acid,isochlorogenic acid C and the fingerprints of the single decoctions and mixed decoctions of Inulae Flos-Haematitum medicinal pair in four groups of proportions.The"peak area/sample weight"value of each common peak in the fingerprints was calculated,and the SPSS 26.0 was used for independent-sample t-test analysis.Results There are significant differences in the"peak area/weight"values of peak 1,peak 2,peak 4,peak 6,peak 9,peak 10,peak 12,peak 13,peak 15 between mixed decoction and single decoction of Inulae Flos-Haematitum medicinal pair with different compatibility ratios(P<0.05),with statistical significance;when the compatibility ratio of Inulae Flos-Haematitum medicinal pair was 3:1,the difference of fingerprints and index components content between single decoction and combined decoction was the largest.Except for peak 7 and peak 14,the difference of"peak area/sample weight"value of other characteristic peaks was statistically significant(P<0.05),and the content difference of 8 index components was statistically significant(P<0.05).Conclusion There are differences in the chemical components of Inulae Flos-Haematitum medicinal pair for single decoction and mixed decoction.