2010-2020年江西省腹泻儿童人星状病毒感染情况及基因型分析

Infection status and genotype of human astrovirus in children with diarrhea in Jiangxi,2010-2020

目的了解2010—2020年江西省腹泻儿童人星状病毒的感染情况以及病毒基因型。方法收集2010—2020年江西省儿童医院15岁以下腹泻儿童的粪便标本,使用实时荧光定量反转录聚合酶链式反应进行人星状病毒核酸检测,对人星状病毒核酸阳性的标本采用反转录聚合酶链式反应对ORF2基因部分区域扩增并进行序列测定分析。结果2010—2020年共采集粪便标本2403份,检出人星状病毒核酸的标本72份,总阳性率为3.00%,5岁以下年龄组的阳性率为3.03%,2岁以下年龄组的感染者占比为84.72%;人星状病毒在同年11月至次年3月的检出率较高;基因测序获得47条病毒ORF2区序列,经比对分析,其中基因1型38条,基因2型1条,基因4型3条,基因5型1条,基因6型4条。结论人星状病毒是引起2岁以下儿童腹泻的重要病原体,2010—2020年江西省流行的星状病毒主要为基因1型,2010年亦存在基因6型的流行。

Objective To understand the infection status and genotypes of human astrovirus in children with diarrhea in Jiangxi province from 2010 to 2020.Methods Stool samples were collected from children with diarrhea under 15 years old in Jiangxi Children's Hospital.The nucleic acid of human astrovirus was detected by real-time RT-PCR.Some regions of ORF2 gene of samples which were positive for astrovirus nucleic acid were amplified and sequenced by RT-PCR.Results A total of 2403 stool samples were collected from 2010 to 2020,in which 72 were astrovirus nucleic acid positive,with a total positive rate of 3.00%.The overall positive rate was 3.03%in age group under 5 years old,and 84.72%of the infected children were under 2 years old.The infection rate of human astrovirus was higher during November-March.Gene sequencing found 47 sequences of the ORF2 region:38 belonged to genotype 1,1 belonged to genotype 2,3 belonged to genotype 4,1 belonged to genotype 5,and 4 belonged to genotype 6.Conclusion Human astrovirus is an important pathogen causing diarrhea in children under 2 years old in Jiangxi.Astrovirus genotype 1 was predominant in Jiangxi from 2010 to 2020,but genotype 6 was detected in 2010.