森林草莓FvbHLH130转录因子调控植株提前开花

The Transcription Factor FvbHLH130 Activates Flowering in Fragaria vesca

分析草莓碱性螺旋—环—螺旋(basichelix-loop-helix,bHLH)转录因子家族,鉴定到森林草莓(Fragaria vesca)花特异表达的转录因子基因FvbHLH130。FvbHLH130编码区序列长度为960 bp,编码319个氨基酸,保守结构域预测结果显示第247~297 aa是bHLH结合域,其属于bHLH家族成员。FvbHLH130启动子含有生长素等激素响应、逆境胁迫和低温响应元件,推测基因表达可能受到生物和非生物胁迫诱导。FvbHLH130蛋白氨基酸序列与月季(Rosa chinensis)的相似性高达93.70%,与其他蔷薇科bHLH蛋白聚在一个分支上,与拟南芥AtFBH4是同源蛋白。农杆菌介导的FvbHLH130拟南芥转基因株系提前7 d开花,且促进开花相关基因AtAP1、AtFT、AtFUL和AtCO的表达。酵母双杂交实验结果表明FvbHLH130与FvARF4和FvARF6互作,可能作为蛋白复合体共同参与开花调控。

The basic helix-loop-helix(bHLH)transcription factor family of strawberry was analyzed,and the transcription factor FvbHLH130 was identified in Fragaria vesca.qRT-PCR analysis in different tissues showed that FvbHLH130 was flower-specifically expressed.Overexpression of FvbHLH130 in Arabidopsis thaliana activated flowering in transgenic lines.The full-length coding sequence of Fvb HLH130was 960 bp,encoding a 319-amino-acid protein.The prediction of conserved domain of Fvb HLH130 showed that 247–297 amino acids were bHLH binding domain.The promoter of FvbHLH130 contained hormone,stress-and low temperature-response elements,which indicated FvbHLH130 might be induced by biotic and abiotic stress.The amino acids sequence of FvbHLH130 revealed up to 93.70%similarity with RcbHLH130.FvbHLH130 sequence was more closely related to Rosaceae bHLH proteins and was homologous to AtFBH4.Agrobacterium-mediated transformation of Arabidopsis thaliana showed that the FvbHLH130 overexpression lines flowered seven days earlier than the control plants,and activated the expression of flowering-related genes AtAP1,AtFT,AtFUL and AtCO.The yeast two-hybrid results showed that FvbHLH130 interacted with FvARF4 and FvARF6,which may form protein complexes in the regulation of flowering.