国兰叶色突变体叶片差异表达基因分析

Analysis of Differential Expression Genes in Leaves of Leaf Color Mutant of Chinese Orchid

中国兰春剑‘隆昌素’(Cymbidium longibracteaturn)及其叶色突变体‘叶艺隆昌素’是研究兰花叶色突变形成机制的良好材料。采用IlluminaHiseq2500对两种材料的叶片进行denovo转录组测序,以此鉴定与叶色突变性状形成相关的差异表达基因。结果表明,色素合成、叶绿体发育相关基因可能是导致‘隆昌素’与‘叶艺隆昌素’叶片性状差异的原因。

The Chinses orchid’Longchangsu’and its leaf color mutant’Yeyi Longchangsu’are excellent materials to study the formation mechanism of the leaf color of orchid.Differentially expressed genes(DEGs)in the leaves of’Longchangsu’and’Yeyi Longchangsu’were identifed by next-generation sequencing using Illumina Hiseq2500.Through the analysis of different expression genes,it was found that the genes related to pigment synthesis and chloroplast development might be the cause of the differences between’Longchangsu’and’Yeyi Longchangsu’.