摘要
目的了解山东省济宁市诺如病毒的分子流行病学特征及诺如病毒的优势基因型,为诺如病毒感染防控提供科学依据。方法按照《山东省病毒性腹泻监测方案》,2018年1月-2020年12月从山东省济宁市每县(市、区)每月采集3~5份病毒性腹泻标本,进行流行病学调查,采用Real time RT-PCR方法检测诺如病毒GI/GⅡ组,对阳性样本进行基因型别鉴定。结果共采集粪便标本1230份,诺如病毒阳性165份(其中GⅠ8份、GⅡ157份),检出率为13.41%(165/1230)。每年除2月份外,其他月份均检出诺如病毒,以6月、7月检出率高。基因型分别为GⅡ.4型25份,占32.05%;GⅡ.2型18份,占23.08%;GⅡ.6型14份,占17.95%;GⅡ.3型13份,占16.67%。3年间优势毒株不同,2018年为GⅡ.3型9份,占69.23%;2019年为GⅡ.2型14份,占43.75%;2020年为GⅡ.4型17份,占53.13%。结论山东省济宁市诺如病毒感染6月和7月流行强度较强,以GⅡ组为主,基因型存在多样性,处于动态变化中,以GⅡ.2[P16]型和GⅡ.4[P31]型为主要型别。
Objective To understand the molecular epidemiological characteristics and genetic diversity of noroviruses infection,so as to provide scientific evidence for the control and prevention.Methods According to the Monitoring Program for Viral Diarrhea in Shandong Province,3-5 samples of viral diarrhea were collected from each county(city,district)in Jining city,Shandong province from January 2018 to December 2020.Epidemiological investigation was performed.Norovirus GI/GⅡgroup was detected by Real time RT-PCR,and the genotype of the positive samples was identified.Results A total of 1230 samples of norovirus infection were detected.The detection rate of norovirus was 13.41%(165/1230).Norovirus was detected in all months of the year except February,with high detection rates in June and July.According to genotyping results,GⅡ.4 accounted for 32.05%(25 samples),GⅡ.2 accounted for 23.08%(18 samples),GⅡ.6 accounted for 17.95%(14 samples),GⅡ.3 accounted for 16.67%(13 samples).The dominant strains differed during the 3 years,with 9 samples of GⅡ.3 type in 2018,accounting for 69.23%;14 samples of GⅡ.2 type in 2019,accounting for 43.75%;and 17 samples of GⅡ.4 type in 2020,accounting for 53.13%.Conclusion Norovirus infections in Jining city,Shandong province are strong in June and July,with group GⅡpredominating,and genotypes are diverse and in dynamic change,GⅡ.2[P16]and GⅡ.4[P31]being the main genotype.
作者
颜丙新
姜文国
傅忠燕
靳淼
YAN Bing-xin;JIANG Wen-guo;FU Zhong-yan;JIN Miao(Seclion of Endemic Disease Control and Prevention,Jining City Center for Disease Control and Prevenlion,Shandong 272000 China;Section of Infectious Disease Control and Prevention,Jining City Center for Disease Control and Prevention;Department of Infectious Disease Control and Prevention,Shandong Center for Disease Control and Prevention;Department of Virus Disease Prevention and Control,Chinese Center for Disease Control and Prevention)
出处
《预防医学论坛》
2023年第2期96-98,共3页
Preventive Medicine Tribune
基金
“十三五”国家科技重大专项(2017ZXl0104001-003-001)
山东预防医学会智飞疾病预防控制技术研究基金项目(LYH2017-13)。
