基于阳离子型铱配合物的黏度荧光探针的设计合成及性质

Design,Synthesis and Properties of Fluorescent Probe based on Cationic Iridium Complex for Detection of Viscosity

以乙基香兰素、1,10-菲罗啉、2-苯基吡啶和三氯化铱为原料,设计合成了一种新型的基于阳离子型铱配合物的荧光探针(LIr).用荧光光谱法研究了探针LIr对黏度的响应性质,结果表明探针LIr识别黏度具有专一性,其荧光强度可增强约140倍.由于探针LIr对细胞的毒性较低,可将其应用于细胞成像研究.

A novel fluorescent probe LIr based on cationic iridium complex was designed and synthesized by using ethyl vanillin,1,10-phenanthroline monohydrate,2-phenylpyridine and IrCl 3 as raw materials.The response of probe LIr to viscosity was investigated by fluorescence spectroscopy.The experimental results indicated that probe LIr could capable of specifically identify the viscosity.Moreover,the fluorescence intensity of probe LIr enhances about 140-fold from water to 99%glycerol.Due to its low cytotoxicity,probe LIr has been successfully applied in cell imaging.