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长裙竹荪β-葡聚糖结构特征和溶液性质 被引量:1

Structural Characterization and Solution Properties ofβ-Glucan from Dictyophora indusiata
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摘要 β-葡聚糖具有多种生理活性和良好的生理功能。长裙竹荪含有较多的β-葡聚糖,目前对其结构与溶液性质缺少系统认识。本文从长裙竹荪中提取一种水溶性多糖(DIPP),采用酸水解、红外光谱、甲基化处理、扫描电镜(SEM)、高效尺寸排阻色谱串联多角度激光散射仪(HPSEC-MALLS)和流变仪等技术对其理化性质、结构特征和溶液性质进行表征。结果表明,DIPP重均分子质量为2.8×10^(6) u,单糖组成仅检测到葡萄糖,糖残基有T-Glap(29.8%)、1,3-Glcp(42.6%)和1,3,6-Glcp(27.8%)。红外光谱显示DIPP具有β构型。DIPP具有较高的特性黏度([η]=1094.3 mL/g),而且0.5%DIPP溶液便表现出较高的黏度和凝胶性质。结论:DIPP是一种在溶液中具有较高黏度的β-葡聚糖,研究结果可为其功能活性研究及产品开发提供参考。 β-glucan has a variety of physiological activities and good physiological functions.Dictyophora indusiata(Vent.Pers.)Fisch.contains lots ofβ-glucan,while its structure and solution properties are not understood clearly.In this paper,a water-soluble polysaccharide(DIPP)was extracted from D.indusiata.The physicochemical properties,structure and solution properties of DIPP were characterized by acid hydrolysis,infrared spectrum,methylation,scanning electron microscope(SEM),high performance size exclusion chromatography multiangle laser light scattering(HPSEC-MALLS)and rheometer.The results showed that the weight average molecular mass of DIPP was 2.8×10^(6) u,and only glucose was detected.The sugar residues were T-Glap(29.8%),1,3-Glcp(42.6%)and 1,3,6-Glcp(27.8%).The infrared spectrum showed that DIPP containedβ-configuration.In dilute solution,DIPP had high intrinsic viscosity([η]=1094.3 mL/g).The DIPP solution(0.5%)showed high viscosity and gel properties.In conclusion,DIPP is aβ-glucan with high viscosity.The results of this study can provide structure basis for the polysaccharide functional activity research and product development.
作者 王喆 殷军艺 聂少平 Wang Zhe;Yin Junyi;Nie Shaoping(Nanchang University,State Key Laboratory of Food Science and Technology,Nanchang 330047)
出处 《中国食品学报》 EI CAS CSCD 北大核心 2023年第3期13-19,共7页 Journal of Chinese Institute Of Food Science and Technology
基金 国家自然科学基金面上项目(31871755)。
关键词 长裙竹荪 Β-葡聚糖 结构 溶液性质 Dictyophora indusiata β-glucan structure solution property
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