摘要
以脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7为体外炎症模型,以促炎症细胞因子白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和炎症介质NO为指标,考察低聚半乳糖(GOS)的体外抗炎活性,并从聚合度的角度探究GOS抗炎活性的构效关系。结果表明,GOS能显著降低促炎症细胞因子(IL-1β,IL-6,TNF-α)和炎症介质NO的分泌,表现出良好的体外抗炎活性。不同聚合度的GOS在调控促炎症细胞因子与炎症介质分泌能力上差异显著,较高聚合度的GOS表现出更强的体外抗炎活性。
Inflammatory mediator(NO)and proinflammatory factors(IL-1β、TNF-αand IL-6)were utilized to evaluate the anti-inflammatory activity of galactooligosaccharide(GOS)in vitro by LPS-induced RAW264.7 macrophages.Moreover,the structure-activity relationship of this bio-activity was also explored from the perspective of the degree of polymerizations.The results showed that GOS could significantly reduce the secretion of inflammatory mediator(NO)and proinflammatory factors(IL-1β、TNF-αand IL-6),implying that GOS had the excellent anti-inflammatory capacity.Besides,the in vitro anti-inflammatory activities of GOS with different degrees of polymerization indicated that there are significant differences in the ability to regulate the secretion of proinflammatory cytokines and inflammatory mediators.In conclusion,GOS with higher degree of polymerization showed stronger anti-inflammatory activity in vitro.
作者
孙聪聪
廖森泰
邹宇晓
庞道睿
刘凡
郝碧芳
Sun Congcong;Liao Sentai;Zou Yuxiao;Pang Daorui;Liu Fan;Hao Bifang(Jiangsu University of Science and Technology,Zhenjiang 212018,Jiangsu;Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Food,Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Products Processing,Guangdong Province,Guangzhou 510600;Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018,Jiangsu)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第3期70-79,共10页
Journal of Chinese Institute Of Food Science and Technology
基金
广东省重点领域研发计划项目(2019B020213001,2020B020225005)
广东省农业科学院农业优势产业学科团队建设项目(202119TD)
现代农业产业技术体系建设专项(CRAS-18)。
关键词
低聚半乳糖
炎症因子
抗炎
聚合度
galactooligosaccharide
inflammatory factor
anti-inflammation
degree of polymerization
