高能电子束辐照对金丝绞瓜贮藏特性的影响

Effects of High Energy Electron Beam Irradiation on Storage Characteristics of Cucurbita pepo L.subsp.Pepo

目的:确定高能电子束辐照处理金丝绞瓜的最优剂量,改善金丝绞瓜的贮藏特性。方法:分别以0.3,0.4 kGy和0.5 kGy剂量的高能电子束从两面处理金丝绞瓜。结果:0.4 kGy高能电子束辐照处理使金丝绞瓜腐烂率降低16.18%,其总酚含量维持较高水平,可溶性固形物含量降低,丙二醛积累量减少,呼吸强度、失重率、色彩饱和度、可滴定酸和过氧化物酶活性均与对照瓜无显著差异(P>0.05),在此剂量下,超氧化物歧化酶的活性高峰是对照瓜的1.14倍,瓜丝细胞超微结构完整;在贮藏120 d时,脆度、葫芦巴碱含量和苯丙氨酸解氨酶活性分别是对照瓜的1.58,3.34倍和1.84倍。结论:0.4 kGy是适宜的高能电子束辐照剂量,有望将其用于金丝绞瓜的贮藏保鲜。

Objective:To determine the optimal dose of high energy electron beam irradiation to treat the Cucurbita pepo L.subsp.Pepo and improve its storage characteristics.Method:This experiment irradiated the two sides of Cucurbita pepo L.subsp.Pepo with high energy electron beams at doses of 0.3,0.4 kGy and 0.5 kGy respectively.Results:0.4 kGy high energy electron beam irradiation can reduce the rotting rate of Cucurbita pepo L.subsp.Pepo by 16.18%,its total phenol content remained at a high level,the soluble solid content decreased,and the accumulation of malondialdehyde decreased.And the respiratory intensity,weight loss rate,color saturation,titratable acid and peroxidase activity were not significantly different from the control melon(P>0.05).At this dose,the peak activity of superoxide dismutase was 1.14 times that of the control melon,and the ultrastructure of the melon silk cell was complete.When stored for 120 days,the brittleness,trigonelline content and phenylalanine ammonia-lyase activity were 1.58,3.34 times and 1.84 times higher than those of the control melon,respectively.Conclusion:So 0.4 kGy irradiated Cucurbita pepo L.subsp.Pepo two sides is the suitable radiation dose,it is expected to be used for the storage and preservation of Cucurbita pepo L.subsp.Pepo.