含ACC脱氨酶的植物促生菌对香石竹生长及瓶插特性的影响

Effects of Plant Growth Promoting Bacteria Containing ACC Deaminase Inoculation on Growth and Vase Features of Carnation

[目的]研究含ACC脱氨酶(1-aminocyclopropane-1-carboxylate deaminase)的不同植物生长促生菌对香石竹生长及瓶插特性的影响。[方法]以香石竹品种Tenie和Master为材料进行盆栽试验,研究接种不同菌株对香石竹生长过程中株高、茎粗、叶绿素相对含量、叶片数、花径和花期以及瓶插过程中鲜质量、叶绿素降解率和叶片凋落的影响。[结果](1)栽培过程中,混合菌株对香石竹生长有一定的促进作用,可显著增加Tenie的叶片数和Master的茎粗,分别较对照高25.0%和6.2%。经SN-2菌株处理后,Master的花期较对照显著延长34.9%。(2)瓶插过程中,与对照相比,所有菌株都可显著抑制Master的叶绿素降解,且效果最好的为SN-2处理,降解率仅为对照的57.6%。经XG菌株处理后,Tenie和Master的鲜质量减少率显著降低,分别仅为对照的79.0%和43.3%。经XG、F和混合菌株处理后,Tenie的叶片凋落率显著降低;而仅有混合菌株可显著抑制Master的叶片凋落,其凋落率仅为对照的54.4%。[结论]栽培中施用混合菌株对香石竹促生效果最好,而在瓶插中XG菌株和混合菌株分别对Tenie和Master采后品质的保持效果最好。

[Purpose]To study the effects of different plant growth promoting bacteria containing ACC deaminase (1-aminocyclopropane-1-carboxylate deaminase) on the growth and vase features of carnation. [Methods]The effects of different inoculated strains on the plant height, stem diameter, chlorophyll relative content, leaf number, flower diameter and flowering period during cultivation, and the fresh weight, chlorophyll degradation rate and litter rate in vase process of carnation Tenie and Master were studied by pot experiment. [ Results] 1) During cultivation, the mixed strains showed some effects, especially increased leaf number of Tenie and stem diameter of Master signific-antly, with 25.0% and 6.2% higher than control, respectively. In addition, the florescence of Master was significantly prolonged by 34.9% after treatment with SN-2. 2) In the vase process, all strains could significantly inhibit the chlorophyll degradation rate of Master, and the SN-2 had the best per-formance, which was only 57.6% of the control. XG strain could significantly reject the decrease of fresh weight of Tenie and Master to 79.0% and 43.3% of control, respectively. XG, F and the mixed strains could significantly decrease the litter rate of Tenie leaves, while only the mixed strains had the same effect on Master leaves, which was only 54.4% of control. [Conclusion]The mixed strains in cultivation have the best growth promotion effect on carnation, while the XG strain and the mixed strains in vase process have the best maintenance effect on the vase characteristics of Tenie and Mas-ter, respectively.