长链非编码RNA TMEVPG1在原发性免疫性血小板减少症患者预后评估中的价值

Value of long non-coding RNA TMEVPG1 in prognosis evaluation of patients with primary immune thrombocytopenia

目的探讨长链非编码RNA(lncRNA)TMEVPG1在原发性免疫性血小板减少症(ITP)患者预后评估中的价值。方法选取126例ITP患者作为研究组,根据ITP患者预后情况分为预后不良组31例和预后良好组95例,另选取同期体检的125例健康者作为对照组。采用实时荧光定量PCR(qRT-PCR)测定lncRNA TMEVPG1相对表达量;采用Pearson法分析ITP患者lncRNA TMEVPG1与血小板计数、干扰素-γ(IFN-γ)、白细胞介素(IL)-2、IL-4、IL-10的相关性;采用COX回归分析探讨影响ITP患者预后的有关因素。结果与对照组比较,研究组患者lncRNA TMEVPG1水平降低,差异有统计学意义(P<0.05);预后不良组患者lncRNA TMEVPG1水平低于预后良好组,差异有统计学意义(P<0.05)。lncRNA TMEVPG1与血小板计数、IL-4、IL-10呈正相关,与IFN-γ、IL-2呈负相关(P<0.05);lncRNA TMEVPG1降低是ITP患者预后不良的独立危险因素(P<0.05)。结论LncRNA TMEVPG1水平降低与ITP患者预后不良有关,其可作为ITP患者预后评估的辅助指标。

Objective To investigate the value of long non-coding RNA(lncRNA)TMEVPG1 in the prognosis evaluation of patients with primary immune thrombocytopenia(ITP).Methods A total of 126 ITP patients were selected as the study group.According to the prognosis of ITP patients,they were divided into poor prognosis group(n=31)and good prognosis group(n=95),and 125 healthy subjects who underwent physical examination during the same period were selected as control group.The relative expression level of lncRNA TMEVPG1 was determined by quantitative real-time polymerase chain reaction(qRT-PCR);correlations of lncRNA TMEVPG1 with platelet count,interferon-γ(IFN-γ),interleukin(IL)-2,IL-4 and IL-10 in ITP patients were explored by Pearson correlation;the factors influencing the prognosis of ITP patients were analyzed by COX regression.Results Compared with the control group,lncRNA TMEVPG1 level in the study group was significantly decreased(P<0.05);lncRNA TMEVPG1 level in the poor prognosis group was significantly lower than that in the good prognosis group(P<0.05).LncRNA TMEVPG1 was positively correlated with platelet count,IL-4 and IL-10,and negatively correlated with IFN-γand IL-2(P<0.05).Lower lncRNA TMEVPG1 was an independent risk factor for poor prognosis in ITP patients(P<0.05).Conclusion The decreased level of LncRNA TMEVPG1 is associated with poor prognosis in ITP patients,which can be used as an auxiliary indicator to evaluate the prognosis of ITP patients.