微小RNA-182-5p和信号转导分子7在翼状胬肉中的表达及临床意义

Expression and clinical significance of microRNA⁃182⁃5p and signal transduction molecule 7 in pterygium

目的检测微小RNA-182-5p(miR-182-5p)和信号转导分子7(SMAD7)在翼状胬肉中的表达情况,探讨二者与患者临床病理特征及预后的相关性。方法选择2018年6月—2021年6月在本院眼科进行手术切除的原发性翼状胬肉组织标本150例进行研究,另选择同期因白内障手术获取的正常球结膜标本60例作为对照。采用实时荧光定量聚合酶链反应(qRT-PCR)检测标本miR-182-5p、SMAD7 mRNA表达水平;采用免疫组织化学染色检测SMAD7蛋白表达水平;采用Pearson法分析翼状胬肉组织中miR-182-5p与SMAD7 mRNA表达水平的相关性;采用双荧光素酶报告基因实验验证miR-182-5p和SMAD7的靶向关系。结果翼状胬肉组织中,miR-182-5p表达水平为(2.26±0.45),高于正常结膜组织的(1.05±0.12);SMAD7 mRNA表达水平为(0.67±0.12),低于正常结膜组织的(1.03±0.15);SMAD7蛋白阳性表达率为23.33%,低于正常结膜组织的71.67%;上述组间差异均有统计学意义(P<0.05)。相关性分析显示,翼状胬肉组织中miR-182-5p与SMAD7 mRNA表达呈负相关(r=-0.536,P<0.05);双荧光素酶报告基因实验结果证实miR-182-5p与SMAD7存在靶向关系;miR-182-5p在进展期表达水平高于静止期,SMAD7在进展期阳性表达低于静止期,差异有统计学意义(P<0.05);与治愈组比较,复发组翼状胬肉组织miR-182-5p表达水平较高,SMAD7 mRNA表达水平及SMAD7阳性表达率较低,差异有统计学意义(P<0.05)。结论翼状胬肉组织中,miR-182-5p呈高表达,SMAD7呈低表达,且二者均与患者临床病理特征和预后有关,或可成为翼状胬肉的治疗靶点。

Objective To detect the expressions of microRNA⁃182⁃5p(miR⁃182⁃5p)and signal transduction molecule 7(SMAD7)in pterygium,and to explore their correlations with clinicopatho⁃logical characteristics and prognosis of patients.Methods A total of 150 primary pterygium tissue specimens removed by surgery in the Department of Ophthalmology of authors′hospital from June 2018 to June 2021 were collected for study,and 60 normal bulbar conjunctiva specimens obtained from cataract surgery in the same period were collected as controls.The expression levels of miR⁃182⁃5p and SMAD7 mRNA in the samples were measured by real⁃time fluorescence quantitative polymerase chain reaction(qRT⁃PCR);the expression level of SMAD7 protein was measured by immunohisto⁃chemical staining;the correlation between miR⁃182⁃5p and expression level of SMAD7 mRNA in pte⁃rygium tissue was analyzed by Pearson method;the dual⁃luciferase reporter gene experiment was used to verify the targeting relationship between miR⁃182⁃5p and SMAD7.Results In pterygium tissue,the expression level of miR⁃182⁃5p was(2.26±0.45),which was higher than(1.05±0.12)in normal conjunctival tissue;the expression level of SMAD7 mRNA was(0.67±0.12),which was lower than(1.03±0.15)in normal conjunctival tissue;the positive expression rate of SMAD7 protein was 23.33%,which was lower than 71.67%in normal conjunctival tissue;the between⁃group differences mentioned above were statistically significant(P<0.05).Correlation analysis showed that the expres⁃sion of miR⁃182⁃5p was negatively correlated with SMAD7 mRNA in pterygium tissue(r=-0.536,P<0.05);the result of dual⁃luciferase reporter gene experiment confirmed that there was a targe⁃ting relationship between miR⁃182⁃5p and SMAD7;the expression level of miR⁃182⁃5p in the pro⁃gressive phase was significantly higher than that in the quiescent phase,and the positive expression of SMAD7 in the progressive phase was significantly lower than that in the quiescent phase(P<0.05);compared with the cured group,the expression level of miR⁃182⁃5p in pterygium tissue in the recurrent group was significantly higher,while the expression level of SMAD7 mRNA and the positive expression rate of SMAD7 were significantly lower(P<0.05).Conclusion In pterygium tissue,the miR⁃182⁃5p is highly expressed while the SMAD7 is low expressed,and both of the two indexes are related to the clinicopathological characteristics and prognosis of patients,which may be⁃come the therapeutic targets of pterygium.