缺铁性贫血儿童的肠道菌群多样性及代谢通路分析

Analysis of intestinal flora diversity and metabolic pathways in children with iron deficiency anemia

[目的]通过高通量测序平台探究缺铁性贫血儿童的肠道菌群有无微生物组成结构的改变,进一步探讨其代谢通路对缺铁性贫血的影响。[方法]选择8例健康儿童(正常组)和5例患有缺铁性贫血儿童(病例组),共13例,收集其静脉血、粪便标本,利用高通量测序后使用扩增子数据分析(QIIME2)等软件对肠道菌群的组成、多样性及代谢通路进行分析。[结果]病例组与正常组在门水平及属水平上的菌群丰富度有明显差异,肠道菌群Alpha和Beta多样性分析发现,正常组的肠道菌群的丰度及多样性高于病例组,且有3种差异性代谢通路。[结论]儿童在缺铁性贫血疾病状态下,肠道菌群在丰度、多样性、代谢通路及菌种方面会发生改变,与正常儿童的肠道菌群相比具有差异性。

[Objective]Through the high-throughput sequencing platform, we explored whether there were any changes in the microbial composition of the intestinal flora of children with iron deficiency anemia.[Methods]Eight healthy children and 5 children with iron deficiency anemia were selected, and their venous blood and feces samples were collected.After high-throughput sequencing, the composition, diversity and metabolic pathways of intestinal flora were analyzed with amplifier data analysis(QIIME2)and other software.[Results]There was a significant difference in the flora abundance at the portal level and genus level between the case group and the normal group.The diversity analysis of the intestinal flora Alpha and Beta showed that the abundance and diversity of the intestinal flora in normal children were higher than those in iron deficiency anemia children, and there were significant differences in the species composition and species of the two intestinal flora.And there were three different metabolic pathways.[Conclusion]In the state of iron deficiency anemia, the intestinal flora changes in terms of abundance, diversity, metabolic pathways and bacterial species, which is different from the intestinal flora of normal children.