西宁地区不同死因雄性大鼠玻璃体液内镁离子、磷离子、尿素、淀粉酶、α羟丁酸脱氢酶的浓度变化与早期死亡时间关系

The relationship between the concentration changes of magnesium ion,phosphorus ion,urea,Amylase and hydroxybutyrate dehydrogenase-αin vitreous fluid of male rats with diffe-rent causes of death and the early death time at Xiningarea

目的探讨西宁地区不同死因雄性大鼠玻璃体液(vitreous humor,VH)内镁离子(Mg^(2+))、磷离子(P^(3+))、尿素(Urea)、淀粉酶(AMS)、α羟基丁酸脱氢酶(α-HBDH)的浓度变化与早期死亡时间(postmortem interval,PMI)的关系。方法将105只雄性大鼠随机分成3个大组(溺死组、勒死组、大失血死亡组),每个大组进一步按照死亡时间(0、3、6、9、12、18、24 h)分为7个小组,每组5只,以每组死后0 h组(死后即刻)作对照组。均置恒温恒湿培养箱(模拟西宁温、湿度:温度为14℃,湿度为55%)。三个死因组于设定的时间点提取玻璃体液,用全自动生化分析检测仪检测Mg^(2+)、P^(3+)、Urea、AMS、α-HBDH浓度。使用SPSS19.0统计软件对相关数据进行统计学分析。结果Mg^(2+)的浓度随着PMI的延长呈现先上升后下降趋势(P<0.05);勒死组和大失血死亡组P^(3+)浓度随PMI延长呈现升高趋势,溺死组P^(3+)浓度随PMI延长呈现先升高后下降趋势;Urea的浓度随PMI延长呈现先上升后下降的趋势(P<0.05);AMS的浓度随着PMI的延长呈现先升高后下降再升高再下降的趋势(P<0.05);α-HBDH的浓度随着PMI的延长呈现先升高后下降再升高的趋势(P<0.05)。不同死因组同一PMI的Mg^(2+)、P^(3+)、Urea、AMS、α-HBDH浓度各不相同。溺死条件下的Mg^(2+)的三次回归模型的r^(2)最大,回归方程:y=0.089+0.004x-0.001x^(2)+2.936E-005x^(3);勒死条件下的Mg^(2+)的三次回归模型的r^(2)最大,回归方程:y=0.062+0.013x-0.001x^(2)+2.410E-005x^(3);大失血死亡条件下的Urea的三次回归模型的r^(2)最大,回归方程:y=0.730-0.011x-0.002x^(2)+6.613E-005x^(3)。结论1.在西宁地区,不同死因雄性大鼠玻璃体液内Mg^(2+)、P^(3+)、Urea、AMS、α-HBDH的浓度变化与早期PMI相关。2.雄性大鼠Mg^(2+)、Urea的浓度随PMI延长呈先升高后下降趋势;α-HBDH浓度随PMI延长呈先升高后下降再升高趋势;溺死条件下的P^(3+)浓度随PMI延长呈先升高后下降趋势,溺死和大失血死亡条件下的P^(3+)浓度与PMI呈正相关;AMS浓度随PMI延长呈先升高后下降再升高再下降的趋势。3.Mg^(2+)、P^(3+)和Urea的三次回归方程更适合于推断溺死条件下的雄性大鼠PMI;AMS的三次回归方程更适合于推断大失血死亡条件下的雄性大鼠PMI;α-HBDH的三次回归方程更适合于推断溺死和勒死条件下的雄性大鼠PMI。

Objective To investigate the relationship between the concentration change of magnesium ion(Mg^(2+)),phosphorus ion(P^(3+)),urea,amylase(AMS),α-hydroxybutyrate dehydrogenase(α-HBDH)in vitreous humor(VH)of male rats with different causes of death and the postmortem interval(PMI)at Xining area.Methods 105 male SD rats were randomly divided into 3 groups(drowning group,strangulation group and severe blood loss death group)for execution.After being killed,they were placed in a constant temperature and humidity incubator(temperature 14C,humidity 55%)to simulate the local temperature and humidity of Xining district.Each group was further divided into 7 groups according to the time of death(0,3,6,9,12,18,24 hours),with 5 rats in each group.The 0 h group after death(immediately after death)as the control group.The constant temperature and humidity incubator(temperature:14℃,humidity:55%,simulating the temperature and humidity of Xining district)was uniformly placed.The vitreous fluid was extracted at the set time point in the three death cause groups and O h in the control group,and Mg^(2+),p^(3+),Urea,AMSα-HBDH concentration.SPSS19.O statistical sofware was used for statistical analysis of relevant data.Results The concentration of Mg^(2+)increased first and then decreased with the prolongation of PMI(P<0.05);The concentration of p^(3+)in strangulation group and severe blood loss death group increased with the prolongation of PMI,while that in drowning group increased first and then decreased with the prolongation of PMI;The urea concentration increased first and then decreased with the prolongation of PMI(P<0.05);The concentration of AMS increased first,then decreased,then increased and then decreased with the prolongation of PMI(P<0.05);The concentration ofα-HBDH increased first,then decreased and then increased with the prolongation of PMI(P<0.05).Mg^(2+),p^(3+),Urea,AMSα-HBDH concentrations.The r^(2)maximum of the cubic regression model of Mg^(2+) under drowning conditions,and the regression equation is:y=0.089+0.004x-0.001x^(2)+2.936E-005x^(3);The r^(2)maximum of the cubic regression model of Mg^(2+) under strangulation condition,and the regression equation is:y=0.062+0.013x-0.001x^(2)+2.410E-005x^(3);The r maximum of the cubic regression model of Urea under death due to massive blood loss condition,and the regression equation is:y=0.730-0.011x-0.002x^(2)+6.613E-005x^(3).Conclusion 1.At Xining area,the concentration changes of magnesium ion,phosphorus ion,urea,amylase andα-HBDH in vitreous fluid of male rats with different causes of death are related to early PMI.2.The concentrations of Mg^(2+)and Urea in male rats increased first and then decreased with the prolongation of PMI;α-HBDH concentration increased first,then decreased and then increased with PMI prolongation;The concentration of p^(3+)under drowning condition increased first and then decreased with the prolongation of PMI,and the concentration of p^(3+)under drowning and massive blood loss death is positively correlated with PMI;The concentration of AMS increased first,then decreased,and then increased and then decreased with the prolongation of PMI.3.The cubic regression equations of Mg^(2+),p^(3+)and Urea are more suitable for inferring PMI of male rats under drowning conditions;The third regression equation of AMS is more suitable for inferring the PMI of male rats under the condition of severe blood loss death;The third regression equation ofα-HBDH is more suitable for inferring PMI of male rats under drowning and strangulation conditions.