肺腺癌转移相关转录本1、Fas相关死亡结构域蛋白、雌激素相关受体α在乳腺癌中的表达及与患者临床分期和预后的关系

Expression of metastasis associated lung adenocarcinoma transcript 1,Fasassociated death domain protein,estrogen-related receptorαin breast cancer and its relationship with clinical stage and prognosis

目的探讨肺腺癌转移相关转录本1(MALAT1)、Fas相关死亡结构域蛋白(FADD)、雌激素相关受体α(ERRα)在乳腺癌中的表达及与患者临床分期和预后的关系。方法选取122例乳腺癌患者,取其乳腺癌组织及相应癌旁组织,逆转录聚合酶链反应(RT-PCR)检测MALAT1的相对表达量,免疫组化法检测FADD、ERRα蛋白的表达情况。比较不同临床分期及预后乳腺癌患者乳腺癌组织中MALAT1、FADD、ERRα的表达情况。绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),评估MALAT1、FADD、ERRα单独及联合检测对乳腺癌患者预后的预测价值。结果乳腺癌组织中MALAT1的相对表达量、ERRα阳性表达率均明显高于癌旁组织(P﹤0.01),FADD阳性表达率明显低于癌旁组织(P﹤0.01)。Ⅲ~Ⅳ期乳腺癌患者乳腺癌组织中MALAT1的相对表达量、ERRα阳性表达率均明显高于Ⅰ~Ⅱ期患者(P﹤0.01),FADD阳性表达率明显低于Ⅰ~Ⅱ期患者(P﹤0.01)。随访1年,死亡23例,生存99例,生存率81.15%(99/122),死亡乳腺癌患者乳腺癌组织中MALAT1的相对表达量、ERRα阳性表达率均明显高于生存患者(P﹤0.01),FADD阳性表达率明显低于生存患者(P﹤0.01)。ROC曲线显示,MALAT1、FADD、ERRα联合检测预测乳腺癌患者预后的AUC为0.872(95%CI:0.813~0.941),高于各指标单独检测。结论MALAT1、FADD及ERRα在乳腺癌组织中异常表达,可能与患者临床分期、预后有关,上述因子联合检测对乳腺癌患者预后的评估价值较高。

Objective To explore the metastasis associated lung adenocarcinoma transcript 1(MALAT1),Fas-associated death domain protein(FADD),estrogen-related receptorα(ERRα)in breast cancer and its relationship with clinical stage and prognosis.Method A total of 122 breast cancer patients were selected,and their breast cancer tissue and corresponding para-cancerous tissue were collected.The relative expression of MALAT1 was detected by reverse transcriptionpolymerase chain reaction(RT-PCR).The expression of FADD and ERRαprotein were detected by immunohistochemical method.The expression of MALAT1,FADD,ERR in breast cancer tissue of breast cancer patients with different clinical stage and prognosis were compared.The receiver operating characteristic(ROC)curve was drawn,and the area under the curve(AUC)was calculated to evaluate the predictive value of MALAT1,FADD,and ERRαalone and in combination on the prognosis of breast cancer patients.Result The relative expression of MALAT1 and the positive rate of ERRαin breast cancer tissue were significantly higher than those in para-cancerous tissue(P<0.01),and the positive rate of FADD was significantly lower than that in para-cancerous tissue(P<0.01).The relative expression of MALAT1 and the positive rate of ERRαin breast cancer tissue of patients with stage III-IV breast cancer tissue were significantly higher than those of stage I-II(P<0.01),and the positive rate of FADD was significantly lower than that of stage I-II(P<0.01).During the one-year follow-up,23 cases died and 99 cases survived,with a survival of 81.15%(99/122).The relative expression of MALAT1 and the positive rate of ERRαin breast cancer tissue of dead breast cancer patients were significantly higher than those of surviving patients(P<0.01),and the positive rate of FADD was significantly lower than that of surviving patients(P<0.01).The ROC curve showed that the AUC of the combined detection of MALAT1,FADD,and ERRαin predicting the prognosis of breast cancer patients was 0.872(95%CI:0.813-0.941),which was higher than that of the individual detection of each index.Conclusion MALAT1,FADD,and ERRαare abnormally expressed in breast cancer tissues.The foregoing factors may be related to the clinical stage and prognosis of patients,and their detection has a high value in the evaluation of the prognosis of breast cancer patients.