蜻蜓凤梨AfHSP基因的分离与功能鉴定

Cloning and Analysis of AfHSP Gene from Aechemia fasciata

蜻蜓凤梨是一种重要的热带花卉,在生产上由于其开花时间、开花率等方面存在较大的不确定性,常用乙烯进行催花,但当温度高于32℃时乙烯的催花效率明显降低,推测可能与相关热激蛋白有关。热激蛋白是生物体中普遍存在的一种调节蛋白,在植物体应对高低温等逆境胁迫时发挥着非常重要的作用,基于蜻蜓凤梨的转录组测序数据和前期对蜻蜓凤梨成花素基因AfFT2(Aechemia fasciata FLOWERING LOCUS T 2)响应乙烯调控开花的研究,筛选出3个可能与开花相关的热激蛋白基因并将其命名为AfHsp20、AfHsp17.6、AfHsp70(Heat shock protein 20,Heat shock protein 17.6,Heat shock protein 70),分别对3个基因进行生物信息学分析、基因功能鉴定及与AfFT2基因启动子的互作实验,初步证实AfHsp20、AfHsp17.6、AfHsp70的表达量受温度影响且响应乙烯,其中AfHsp17.6能与AfFT2启动子发生互作。本研究为进一步研究高温条件下乙烯催花的作用机制提供参考,也为解决高温环境中凤梨科植物催花问题提供相关理论依据。

Aechemia fasciata is an important tropical flower.Due to the large uncertainties in its flowering time and rate,ethylene is often used to promote flowers in production.However,the flower urging efficiency of ethylene is significantly reduced when the temperature is higher than 32℃,HSP20,HSP17.6 and HSP70(Heat shock protein 20,Heat shock protein 17.6,Heat shock protein 70)genes play an important role in plant growth and development and in response to heat stress and low temperature.This study is based on the transcriptome sequencing data of the Aechemia fasciata and the previous research on the regulation of AfFT2(Aechemia fasciata FLOWERING LOCUS T 2)in response to ethylene.Three heat shock protein genes that may be related to flowering were selected and named as AfHsp20,AfHsp17.6 and AfHsp70.The three genes were analyzed by bioinformatics,gene function identification and interaction experiments.The study initially confirmed that the expression of AfHsp20,AfHsp17.6 and AfHsp70 was affected by temperature and in response to ethylene,in which AfHsp17.6 could interact with the AfFT2 promoter.This study provided a reference for further research on the mechanism of ethylene to promote flowering under high temperature conditions and relevant theoretical basis for solving the flower forcing problem of bromeliaceae plants in high temperature environment.