敲减PPARG对乳腺癌MCF-7细胞裸鼠移植瘤生长影响的研究

Effect of PPARG knockdown on the growth of breast cancer MCF-7 cell xenografted tumor in nude mice

目的:探讨敲减过氧化物酶体增殖物激活受体γ(PPARG)对乳腺癌MCF-7细胞裸鼠移植瘤生长的影响。方法:体外培养MCF-7细胞,将PPARG-siRNA和NC-siRNA转染进入MCF-7细胞。分别采用CCK-8法和流式细胞术检测细胞增殖和凋亡情况。将转染后的细胞建立裸鼠移植瘤模型。28 d后处死裸鼠,分离瘤体并称重,计算抑瘤率。采用RT-PCR和蛋白印迹法分别检测瘤体中PPARG、β-catenin和MMP-9的mRNA表达和蛋白水平。结果:与空白对照组比较,NC-siRNA组各指标比较差异无统计学意义(P>0.05);与空白对照组和NC-siRNA组比较,PPARG-siRNA组MCF-7细胞增殖率降低、细胞凋亡率增加(P<0.05);与空白对照组和NC-siRNA组比较,PPARG-siRNA组移植瘤质量减少,PPARG、NF-κB和MMP-9的mRNA表达和蛋白水平均降低,抑瘤率增加(P<0.05)。结论:敲减PPARG能抑制乳腺癌MCF-7细胞增殖,促进MCF-7细胞凋亡,抑制裸鼠移植瘤生长,发生机制可能与PPARG调控能量代谢有关。

Objective:To investigate the effect of PPARG knockdown on the growth of breast cancer MCF-7 cell xenografted tumor in nude mice.Methods:MCF-7 cells were cultured in vitro.PPARG siRNA and NC siRNA virus suspension were transfected into MCF-7 cells.Cell proliferation and apoptosis were detected by CCK-8 method and flow cytometry.The transfected cells were used to establish transplanted tumor model.After 28 days,the nude mice were killed,the tumor body was separated,the tumor body weighed,and the tumor inhibition rate was calculated.RT-PCR and Western blotting were used to detect PPARG,β-catenin and MMP-9 in tumor.Results:Compared with blank control group,the various indicators in NC-siRNA group had no significant changes(P>0.05).Compared with blank control group and NC-siRNA group,the MCF-7 cell proliferation rate of PPARG-siRNA group decreased,the rate of cell apoptosis increased(P<0.05).Compared with blank control group and NC-siRNA group,the tumor weight,PPARG,NF-κB and MMP-9 mRNA and protein in the PPARG-siRNA group decreased,and the tumor inhibition rate increased(P<0.05).Conclusion:PPARG knockdown can inhibit the proliferation of MCF-7 cells and promote the apoptosis of MCF-7 cells,and inhibit the growth of transplanted tumor in nude mice,which may be related to the regulation of energy metabolism by PPARG.