OGDHL与BRCA2蛋白在胃癌组织中的表达及其临床意义

Expression of OGDHL and BRCA2 protein in gastric cancer and its clinical significance

目的:探讨类草酸谷氨酸脱氢酶(OGDHL)与乳腺癌2号基因(BRCA2)蛋白在胃癌组织中的表达及其临床意义。方法:选取本院2016年6月至2018年12月收治的136例胃癌患者癌组织(病例组)及癌旁组织(癌旁组)为研究对象,采用实时荧光逆转录(RT-PCR)及免疫组织化学法检测胃癌组织和癌旁组织中OGDHL、BRCA2 mRNA及蛋白水平,采用χ2检验及Cox回归分析胃癌患者临床病理特征、预后与OGDHL、BRCA2表达的相关性。结果:病例组OGDHL、BRCA2 mRNA表达低于癌旁组(P<0.05)。病例组OGDHL、BRCA2蛋白水平和阳性率低于癌旁组(P<0.05)。OGDHL、BRCA2蛋白水平与胃癌患者年龄无关(P>0.05),与肿瘤最大径、TNM分期、浸润深度、淋巴血管间隙浸润、淋巴结转移、复发相关,且肿瘤最大径≥5 cm、TNM分期越高、浸润深度越深、有淋巴血管间隙浸润、淋巴结转移、复发的患者OGDHL、BRCA2蛋白阳性表达率越低(P<0.05)。OGDHL、BRCA2阳性组生存率明显高于OGDHL、BRCA2阴性组(P<0.05)。OGDHL、BRCA2阴性表达是影响胃癌患者预后的独立危险因素(HR=2.99、4.30,95%CI:1.92~4.56、2.03~9.13,P<0.05)。结论:胃癌组织中OGDHL、BRCA2表达减少,其表达水平与胃癌临床病理特征相关,OGDHL、BRCA2阴性表达是胃癌预后的危险因素。

Objective:To investigate the expression of Oxalate-like glutamate dehydrogenase(OGDHL)and Breast cancer 2 gene(BRCA2)protein in gastric cancer and its clinical significance.Methods:136 patients with gastric cancer tissues(case group)and adjacent normal specimens(para-cancerous tissues)admitted to our hospital from June 2016 to December 2018 as the research objects were selected.RT-PCR and immunohistochemistry were used to analyze the mRNA and protein levels of OGDHL and BRCA2 in gastric cancer tissues and adjacent tissues.Chi square test and Cox regression analysis were used to explore the correlation between clinicopathological features,prognosis and OGDHL,BRCA2.Results:The expression levels of OGDHL and BRCA2 mRNA in the case group were lower than those in the adjacent group(P<0.05).The OGDHL and BRCA2 protein expression scores and positive rates in the case group were lower than those in the adjacent group(P<0.05).The expression of OGDHL and BRCA2 protein was not correlated with the age of patient(P>0.05),but correlated with the largest tumor diameter,TNM stage,depth of invasion,lymphatic vascular space infiltration,lymph node metastasis,and recurrence,and the largest tumor diameter≥5 cm,the higher the TNM stage,the deeper the infiltration depth,the lymphatic and vascular space infiltration,the lymph node metastasis,recurrence,the lower the positive expression rate of OGDHL and BRCA2 protein(P<0.05).The survival rate of OGDHL and BRCA2 positive group were significantly higher than those of OGDHL and BRCA2 negative group(P<0.05).The negative expression of OGDHL and BRCA2 were independent risk factors affecting the prognosis of gastric cancer patients(HR=2.99,4.30,95%CI:1.92~4.56,2.03~9.13,P<0.05).Conclusion:The expression of OGDHL and BRCA2 in gastric cancer tissues is reduced,and the expression levels of OGDHL and BRCA2 are related to the clinicopathological characteristics of gastric cancer.The negative expression of OGDHL and BRCA2 are risk factors for the prognosis of gastric cancer.