小麦淀粉对粗壮女贞苦丁茶提取物的负载、活性保护及缓释作用

Loading,Activity Protection and Sustained-Release Effect of Wheat Starch on Ligustrum robustum(Rxob.)Blume Extract

目的:研究小麦淀粉对粗壮女贞苦丁茶提取物(Ligustrum robustum(Rxob.)Blume extract,LRE)的负载、抗氧化活性保护及缓释能力,为其在食品及生物医药行业的高附加值开发利用提供理论基础。方法:采用质谱、核磁共振波谱鉴定LRE主要成分,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、还原能力等体外抗氧化方法测定LRE体外抗氧化能力,以总酚累计释放量表征淀粉对LRE负载能力及负载稳定性,并分析淀粉对LRE抗氧化活性的保护作用,探究模拟体外消化过程淀粉对LRE的缓释作用。结果:经鉴定LRE主要活性成分为粗壮女贞苷B(C_(31)H_(44)O_(13))、粗壮女贞苷N(C_(35)H_(46)O_(18))和紫茎女贞苷J(C_(35)H_(46)O_(19)),具有较强的DPPH自由基清除能力和还原能力,不同添加量的小麦淀粉可以较为稳定地负载LRE,一定程度上延缓了LRE的自由基清除能力和还原能力的衰减以及在体外模拟消化系统中的释放。

Purpose:To determine the loading,antioxidant activity protection and sustained-release effect of wheat starch on Ligustrum robustum(Rxob.)Blume extract(LRE)for the purpose of providing a theoretical basis for the high value added development and utilization of LRE in the food and biomedical industries.Methods:The major compounds in LRE were identified by mass spectroscopy(MS)and nuclear magnetic resonance(NMR)spectroscopy,and the in vitro antioxidant capacity of LRE was determined by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity and reducing power assays.The loading capacity and stability of starch for LRE were measured by cumulative release of total phenols.Besides,the protective effect of starch on the antioxidant activity of LRE was analyzed,and the sustained-release effect of starch on LRE during in vitro simulated digestion was investigated.Results:The major bioactive compounds in LRE were identified as ligurobustoside B(C_(31)H_(44)O_(13)),ligurobustoside N(C_(35)H_(46)O_(18))and ligupurpuroside J(C_(35)H_(46)O_(19)),and LRE had strong free radical scavenging capacity and reducing power.Furthermore,different concentrations of wheat starch could stably load LRE,delaying the attenuation of the free radical scavenging capacity and reducing power of LRE and inhibiting the release of LRE during in vitro simulated digestion to a certain extent.