两种大鼠高脂血症模型的特点及性别差异研究

Characteristics and sex differences of two kinds of hyperlipidemia models in rats

目的考察两种大鼠高脂血症模型体重、摄食量、血脂变化趋势及肝组织病理变化,比较不同性别高脂血症模型的差异。方法大鼠采用两种高脂饲料喂养7~8周,建立混合型高脂血症模型和高胆固醇血症模型。造模期间记录动物体重、摄食量和一般状态,分别于第0、1~2、7~8周检测分析血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C),高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)含量变化;造模后取动物的肝进行组织病理学观察。结果两模型高脂饲料喂养组比较,混合型高脂血症体重显著高于空白对照组,摄食量显著降低,总食物利用度升高,而高胆固醇血症摄食量升高,体重及总食物利用率与空白对照组无显著差异;造模结束时混合型高脂血症TC、TG水平升高,高胆固醇血症TC升高,TG不变,混合型高脂血症肝脂肪变性程度更强。同模型下雄性总体血脂变化、肝脂肪变性程度均重于雌性。结论两模型高脂饲料喂养组在体重、摄食量、血脂水平及肝病理观察均有差异,同模型雄性程度强于雌性。

ObjectiveTo investigate the trend in changes of body weight,ingestion,blood lipids,and pathology of liver tissue in two models of hyperlipidemia in rats,and to compare differences of the hyperlipidemia models of different genders.MethodsRats were fed two kinds of high fat diet for 7~8 weeks to establish models of mixed hyperlipidemia and hypercholesterolemia.The body weight,ingestion,and general state of the animals were recorded during the modeling period.Changes in serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)were detected and analyzed at 0,1~2 and 7~8 weeks.ResultsThe body weight of the mixed hyperlipidemia high-fat diet group was significantly higher than control group,ingestion was decreased significantly,and total food use was increased,while ingestion of hypercholesterolemia high-fat diet group was increased and there was no significant difference in body weight or total food use.At the end of modeling,TC and TG levels in the mixed hyperlipidemia groups was increased,the TC level in the hypercholesterolemia group was increased,TG was unchanged,and the degree of hepatic steatosis in the mixed hyperlipidemia group was stronger.In the same model,the overall change in blood lipids and the degree of liver steatosis in male rats were more severe than those in female rats.ConclusionsThere were differences in body weight,ingestion,lipid levels and liver pathology in high fat diet groups of the two models with a stronger degree in male than in female rats of the same model.