新加达原散联合抗生素对临床分离多重耐药大肠埃希菌浮游状态作用趋势研究

Study on Effect Trend of NDYP Combined with Antibiotics on Planktonic Status of Clinically Isolated Multi-drug Resistant Escherichia coli

目的探索新加达原散联合常用抗生素对临床分离多重耐药大肠埃希菌浮游状态的干预作用。方法实验以筛选出的产ESBLs酶且成膜能力最强的前5株大肠埃希菌为研究对象,通过MTT、棋盘法探索新加达原散联合常用抗生素对细菌浮游状态的影响。结果新加达原散联合常用抗生素用药对成膜能力最强的前5株具有协同或相加作用。结论体外研究揭示了新加达原散联合常用抗生素对浮游状态临床分离多重耐药大肠埃希菌具有抑制作用,新加达原散具有增敏和协同抗生素抑菌的作用。

Objective To explore the intervention effect of New DaYuan Powder(NDYP)combined with common antibiotics on the floating status of multi-drug resistant Escherichia coli isolated from patients.Methods The first 5 strains of Escherichia coli which produced extended-spectrum beta-lactamases(ESBLs)and had the best ability of forming membrane were selected as the research objects,the effect of NDYP combined with common antibiotics on the status of bacterial plankton was determined by(MTT)and Chess-Board methods.Results The results showed that the combination of NDYP and common antibiotics had synergistic or additive effects on the first 5 strains with the strongest membrane-forming ability.Conclusion This in vitro study shows that the combination of NDYP and common antibiotics has an inhibitory effect on floating multi-drug resistant Escherichia coli,and NDYP can enhance the sensitivity and synergize with antibiotics.