摘要
通过不同的修饰方法制备了以γ-Fe_(2)O_(3)为核的四种氨基化的磁性纳米材料分别为γ-Fe_(2)O_(3)@SiO2-cysteamine, γ-Fe_(2)O_(3)@SiO2-PEI, γ-Fe_(2)O_(3)@SiO2-G1.0, γ-Fe_(2)O_(3)@SiO2-APTMS.在吸附时间为0.5, 1, 2,4, 8 h, p H=3, 5, 7, 9, 11的条件下,通过磁固相萃取的方法吸附浓度为2.5×10^(-6) mol/L的苯丙氨酸,随后用荧光分光光度计检测上清液的荧光强度,荧光强度越低说明纳米材料的吸附效果越优秀.通过试验后发现γ-Fe_(2)O_(3)@SiO2-G1.0磁性纳米粒子在p H=5,吸附时间为8 h的条件下的吸附性最好. γ-Fe_(2)O_(3)@SiO2-PEI磁性纳米粒子在pH=3,吸附时间为0.5 h条件下的吸附性略逊于上者,但该材料的制备过程简易,吸附氨基酸所需时间更短.因此功能化的γ-Fe_(2)O_(3)磁性纳米材料用于固相萃取氨基酸具有一定的研究前景.
Four aminogenized nanoparticles were prepared as follows: γ-Fe_(2)O_(3)@SiO2-cysteamine, γ-Fe_(2)O_(3)@SiO2-APTMS, γ-Fe_(2)O_(3)@SiO2-PEI, γ-Fe_(2)O_(3)@SiO2-G1.0. Magnetic solid phase extraction experiments of L-phenylalanine have been carried out at a concentration of 2.5×10^(-6)mol/L, different extraction times and p H gradients. The lower fluorescence intensity of supernatant indicated the better extract effects.As for γ-Fe_(2)O_(3)@SiO2-G1.0 nanoparticles, the best adsorption was obtained at p H=5, while the adsorption time was 8 h. As for γ-Fe_(2)O_(3)@SiO2-PEI nanoparticles, less adsorption than above could be obtained at p H=3 and 0.5 h. γ-Fe_(2)O_(3)@SiO2-PEI nanoparticles took less time to extract L-phenylalanine. Functionalized γ-Fe_(2)O_(3)nanoparticles are promising for solid-phase extraction of L-phenylalanine.
作者
秦铭杉
朱欣怡
韦冬微
宋淇淇
金天明
李存
杨琳燕
Qin Mingshan;Zhu Xinyi;Wei Dongwei;Song Qiqi;Jin Tianming;Li Cun;Yang Linyan(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Academy of Agricultural Sciences,Tianjin,300192,China)
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第1期102-111,共10页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
Supported by Outstanding Research Achievement Award Project of Tianjin Agricultural University
the Open Topic of Guangxi Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (CMEMR2016-B12)
the National Natural Science Foundation of China (21875117, 31572492)
the Municipal-level Innovative and Entrepreneurial Training Plan for College Students (202210061109)
the Veterinary Bitechnology Scientific Research Innovation Team of Tianjin,China (TD13-5091)
the Veterinary“Innovative Talent People of Young and Middle Age Key Members Project”of Tianjin
the Natural Science Foundation of Tianjin,China (18JCYBJC30100, 19JCQNJC13700)。
